Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kempe, K.; Higashi, Y.; Frick, S.; Sabarna, K.; Kutchan, T.M.
    RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes (2009), Phytochemistry, 70, 579-589.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.248 cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL Papaver somniferum
2.3.1.150 expressed in Escherichia coli as a His-tagged fusion protein Papaver somniferum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.248 additional information direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident Papaver somniferum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.248 cytosol
-
 Papaver somniferum 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.150 additional information Papaver somniferum yeast two-hybrid and co-immunoprecipitation analyses indicate an interaction between salutaridine reductase and SalAT, which suggest the occurrence of an enzyme complex ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.248 Papaver somniferum
-
-
-
2.3.1.150 Papaver somniferum Q94FT4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.248 His-tagged SalR purified with a cobalt affinity resin Papaver somniferum
2.3.1.150 using affinity chromatography and gel filtration Papaver somniferum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.248 leaf
-
 Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.248 salutaridine + NADPH + H+ interaction between SalR and SalAT in morphine biosynthesis Papaver somniferum (7S)-salutaridinol + NADP+
-
 ?
2.3.1.150 additional information yeast two-hybrid and co-immunoprecipitation analyses indicate an interaction between salutaridine reductase and SalAT, which suggest the occurrence of an enzyme complex Papaver somniferum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.248 SalR
-
 Papaver somniferum
2.3.1.150 SalAT
-
 Papaver somniferum
2.3.1.150 salutaridinol 7-O-acetyltransferase
-
 Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.248 NADPH
-
 Papaver somniferum

General Information

EC Number General Information Comment Organism
2.3.1.150 malfunction RNA interference of salAT leads to accumulation of the intermediate compounds, salutaridine and salutaridinol. SalAT transcript is shown by quantitative PCR to be diminished, while salutaridine reductase transcript levels remains unaffected Papaver somniferum
2.3.1.150 physiological function yeast two-hybrid and co-immunoprecipitation analyses indicate an interaction between salutaridine reductase and SalAT, which suggest the occurrence of an enzyme complex Papaver somniferum