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Literature summary extracted from

  • K๖llner, T.G.; Gershenzon, J.; Degenhardt, J.
    Molecular and biochemical evolution of maize terpene synthase 10, an enzyme of indirect defense (2009), Phytochemistry, 70, 1139-1145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.47 expressed in Escherichia coli Zea mays
4.2.3.47 expressed in Escherichia coli Zea diploperennis
4.2.3.47 expressed in Escherichia coli Zea perennis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.47 additional information generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per Zea mays
4.2.3.47 additional information generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per Zea diploperennis
4.2.3.47 additional information generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per Zea perennis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.47 (2E,6E)-farnesyl diphosphate Zea mays maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate Zea diploperennis maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate Zea perennis maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives (E)-beta-farnesene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.47 Zea diploperennis C7E5V9
-
-
4.2.3.47 Zea mays C7E5V7 subsp. mexicana
-
4.2.3.47 Zea mays C7E5V8 subsp. huehuetenangensis
-
4.2.3.47 Zea mays Q2NM15
-
-
4.2.3.47 Zea perennis C7E5W0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.47
-
 Zea mays
4.2.3.47
-
 Zea diploperennis
4.2.3.47
-
 Zea perennis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.47 (2E,6E)-farnesyl diphosphate
-
 Zea mays (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate
-
 Zea diploperennis (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate
-
 Zea perennis (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea mays (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea diploperennis (E)-beta-farnesene + diphosphate
-
 ?
4.2.3.47 (2E,6E)-farnesyl diphosphate maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea perennis (E)-beta-farnesene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.47 terpene synthase 10
-
 Zea mays
4.2.3.47 terpene synthase 10
-
 Zea diploperennis
4.2.3.47 terpene synthase 10
-
 Zea perennis
4.2.3.47 terpene synthase 10-B73
-
 Zea mays
4.2.3.47 terpene synthase 10-per
-
 Zea perennis
4.2.3.47 TPS10
-
 Zea mays
4.2.3.47 TPS10
-
 Zea diploperennis
4.2.3.47 TPS10
-
 Zea perennis
4.2.3.47 TPS10-dip
-
 Zea diploperennis

Expression

EC Number Organism Comment Expression
4.2.3.47 Zea mays induced by herbivory up
4.2.3.47 Zea diploperennis induced by herbivory up
4.2.3.47 Zea perennis induced by herbivory up

General Information

EC Number General Information Comment Organism
4.2.3.47 physiological function maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea mays
4.2.3.47 physiological function maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea diploperennis
4.2.3.47 physiological function maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives Zea perennis