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Literature summary extracted from

  • Ynalvez, R.A.; Xiao, Y.; Ward, A.S.; Cunnusamy, K.; Moroney, J.V.
    Identification and characterization of two closely related beta-carbonic anhydrases from Chlamydomonas reinhardtii (2008), Physiol. Plant., 133, 15-26.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.1 2-mercaptoethanol increases the activity of isozyme CAH7 by 69% but has no effect on the activity of isozyme CAH8 Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide
-
 Chlamydomonas reinhardtii
4.2.1.1 azide
-
 Chlamydomonas reinhardtii
4.2.1.1 cyanide
-
 Chlamydomonas reinhardtii
4.2.1.1 ethoxyzolamide
-
 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 periplasm
-
 Chlamydomonas reinhardtii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ zinc metalloenzyme Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 40000
-
 2 * 40000, mature isozyme CAH8 Chlamydomonas reinhardtii
4.2.1.1 80000
-
 isoform CAH8, SDS-PAGE Chlamydomonas reinhardtii
4.2.1.1 84000
-
 isoform CAH7, SDS-PAGE Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Chlamydomonas reinhardtii
-
-
-
4.2.1.1 Chlamydomonas reinhardtii D66
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 amylose resin chromatography Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
 the recombinant CAH7 protein has a specific activity of 3.1 Wilbur Anderson units/mg, while the recombinant CAH8 protein has an activity of 4.2 Wilbur Anderson units/mg Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Chlamydomonas reinhardtii H2CO3
-
 r
4.2.1.1 CO2 + H2O
-
 Chlamydomonas reinhardtii D66 H2CO3
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 homodimer 2 * 40000, mature isozyme CAH8 Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-carbonic anhydrase
-
 Chlamydomonas reinhardtii
4.2.1.1 CAH7 isoform Chlamydomonas reinhardtii
4.2.1.1 CAH8 isoform Chlamydomonas reinhardtii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.003
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii azide
4.2.1.1 0.0044
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii acetazolamide
4.2.1.1 0.015
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii cyanide
4.2.1.1 0.02
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii ethoxyzolamide
4.2.1.1 0.041
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii acetazolamide
4.2.1.1 0.044
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii ethoxyzolamide
4.2.1.1 0.061
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii cyanide
4.2.1.1 1.9
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii azide