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Literature summary extracted from

  • Plancarte, A.; Nava, G.; Mendoza-Hernandez, G.
    Purification, properties, and kinetic studies of cytoplasmic malate dehydrogenase from Taenia solium cysticerci (2009), Parasitol. Res., 105, 175-183.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 arsenate uncompetitive inhibition Taenia solium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0024
-
 oxaloacetate
-
 Taenia solium
1.1.1.37 0.048
-
 NADH
-
 Taenia solium
1.1.1.37 0.05
-
 NAD+
-
 Taenia solium
1.1.1.37 0.215
-
 (S)-malate
-
 Taenia solium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Taenia solium 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 32000
-
 2 * 32000, native enzyme, gel filtration Taenia solium
1.1.1.37 64000
-
 native enzyme, gel filtration Taenia solium

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Taenia solium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 ammonium sulfate fractionation, CM-Sepharose column chromatography, and Blue Sepharose column chromatography Taenia solium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 cysticercus
-
 Taenia solium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 0.054
-
 crude extract, in the reduction of oxaloacetate Taenia solium
1.1.1.37 2.615
-
 48.3fold purified enzyme, in the reduction of oxaloacetate Taenia solium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Taenia solium oxaloacetate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 homodimer 2 * 32000, native enzyme, gel filtration Taenia solium

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 cMDH
-
 Taenia solium
1.1.1.37 L-malate: NAD oxidoreductase
-
 Taenia solium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 10 45 the parasite enzyme activity is not affected in a broad temperature range (from 10°C to 45°C), the enzyme is also stable at 20°C and 37°C over a 30 min period Taenia solium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.6
-
 for oxaloacetate reduction Taenia solium
1.1.1.37 9.6
-
 for (S)-malate oxidation Taenia solium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.37 6.8 8.5 in the forward reaction Taenia solium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 additional information can also use 3-acetylpyridine-adenine and nicotinamide guanine as cofactors but with less specificity than NAD+ Taenia solium
1.1.1.37 NAD+
-
 Taenia solium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.37 8.2
-
 arsenate for the oxaloacetate reduction Taenia solium
1.1.1.37 77
-
 arsenate for the malate oxidation Taenia solium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.37 Taenia solium isoelectric focusing
-
 8.7