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Literature summary extracted from

  • Haghighatian, M.; Mofid, M.R.; Nekouei, M.K.; Yaghmaei, P.; Tafreshi, A.H.
    Isomalt production by cloning, purifying and expressing of the MDH gene from Pseudomonas fluorescens DSM 50106 in different strains of E. coli (2008), Pak. J. Biol. Sci., 11, 2001-2006.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.67 industry best strain for expression of MDH in both laboratory and industrial applications is Escherichia coli BL21 (DE3) plysS Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.67 subcloned into vector pDEST110 and overexpressed in different strains of Escherichia coli (BL21 (DE3) plysS, JM109, Origami(DE3) or M15) Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 55000
-
x * 55000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Pseudomonas fluorescens
-
DSM 50106
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67 by Ni2+-NTA affinity chromatography Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 63
-
MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 arabitol + NAD(P)+
-
Pseudomonas fluorescens ? + NAD(P)H
-
?
1.1.1.67 D-fructose + NAD(P)+
-
Pseudomonas fluorescens ? + NAD(P)H
-
?
1.1.1.67 isomaltulose + NAD(P)+
-
Pseudomonas fluorescens ? + NAD(P)H
-
?
1.1.1.67 additional information among the Escherichia coli strains, BL21 (DE3) plysS exhibits the maximum expression level of MDH (11mg/L) Pseudomonas fluorescens ?
-
?
1.1.1.67 sorbitol + NAD(P)+
-
Pseudomonas fluorescens ? + NAD(P)H
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.67 ? x * 55000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.67 MDH
-
Pseudomonas fluorescens
1.1.1.67 NADH-dependent mannitol dehydrogenase
-
Pseudomonas fluorescens