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Literature summary extracted from

  • Kandeel, M.; Nakanishi, M.; Ando, T.; El-Shazly, K.; Yosef, T.; Ueno, Y.; Kitade, Y.
    Molecular cloning, expression, characterization and mutation of Plasmodium falciparum guanylate kinase (2008), Mol. Biochem. Parasitol., 159, 130-133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.8 DNA and amino acid sequence determination and analysis, sequence comparison Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.8 D74A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
2.7.4.8 E101D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
2.7.4.8 N103D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
2.7.4.8 Y76F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 AMP 10-15% inhibition at 5 mM Plasmodium falciparum
2.7.4.8 CMP 10-15% inhibition at 5 mM Plasmodium falciparum
2.7.4.8 dAMP 10-15% inhibition at 5 mM Plasmodium falciparum
2.7.4.8 dCMP 10-15% inhibition at 5 mM Plasmodium falciparum
2.7.4.8 dGMP competitive versus GMP and mixed-type versus ATP Plasmodium falciparum
2.7.4.8 EDTA 85% inhibition at 3 mM Plasmodium falciparum
2.7.4.8 additional information no inhibition by TMP and by 2-mercaptoethanol up to 5 mM Plasmodium falciparum
2.7.4.8 UMP 10-15% inhibition at 5 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 additional information
-
additional information kinetics of wild-type and mutant enzymes Plasmodium falciparum
2.7.4.8 0.0222
-
GMP wild-type enzyme Plasmodium falciparum
2.7.4.8 0.0592
-
GMP mutant Y76F Plasmodium falciparum
2.7.4.8 0.0616
-
GMP mutant E101D Plasmodium falciparum
2.7.4.8 0.0731
-
dGMP mutant Y76F Plasmodium falciparum
2.7.4.8 0.0746
-
dGMP wild-type enzyme Plasmodium falciparum
2.7.4.8 0.1816
-
dGMP mutant D74A Plasmodium falciparum
2.7.4.8 0.1894
-
dGMP mutant E101D Plasmodium falciparum
2.7.4.8 0.1933
-
GMP mutant D74A Plasmodium falciparum
2.7.4.8 0.2265
-
GMP mutant N103D Plasmodium falciparum
2.7.4.8 0.2364
-
dGMP mutant N103D Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.8 ATP + GMP Plasmodium falciparum
-
ADP + GDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Plasmodium falciparum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 30
-
with substrates ATP and dGMP Plasmodium falciparum
2.7.4.8 750
-
with substrate ATP and GMP Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + AMP very low activity with AMP Plasmodium falciparum ADP + ADP
-
?
2.7.4.8 ATP + dGMP low activity, dGMP acts also as inhibitor Plasmodium falciparum ADP + dGDP
-
?
2.7.4.8 ATP + GMP
-
Plasmodium falciparum ADP + GDP
-
?
2.7.4.8 additional information no activity with CMP, dTMP, dAMP, dCMP, and UMP Plasmodium falciparum ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.8 2 8 dGMP mutant Y76F Plasmodium falciparum
2.7.4.8 26.5
-
dGMP mutant E101D Plasmodium falciparum
2.7.4.8 26.8
-
dGMP mutant N103D Plasmodium falciparum
2.7.4.8 33.1
-
dGMP mutant D74A Plasmodium falciparum
2.7.4.8 43
-
dGMP wild-type enzyme Plasmodium falciparum
2.7.4.8 848
-
GMP mutant E101D Plasmodium falciparum
2.7.4.8 946
-
GMP wild-type enzyme Plasmodium falciparum
2.7.4.8 1032
-
GMP mutant Y76F Plasmodium falciparum
2.7.4.8 1080
-
GMP mutant N103D Plasmodium falciparum
2.7.4.8 1121
-
GMP mutant D74A Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7.2 7.8
-
Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.8 6 9
-
Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.148
-
dGMP versus GMP Plasmodium falciparum
2.7.4.8 0.4
-
dGMP versus ATP Plasmodium falciparum