EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.8 | DNA and amino acid sequence determination and analysis, sequence comparison | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.8 | D74A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Plasmodium falciparum |
2.7.4.8 | E101D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Plasmodium falciparum |
2.7.4.8 | N103D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Plasmodium falciparum |
2.7.4.8 | Y76F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.8 | AMP | 10-15% inhibition at 5 mM | Plasmodium falciparum | |
2.7.4.8 | CMP | 10-15% inhibition at 5 mM | Plasmodium falciparum | |
2.7.4.8 | dAMP | 10-15% inhibition at 5 mM | Plasmodium falciparum | |
2.7.4.8 | dCMP | 10-15% inhibition at 5 mM | Plasmodium falciparum | |
2.7.4.8 | dGMP | competitive versus GMP and mixed-type versus ATP | Plasmodium falciparum | |
2.7.4.8 | EDTA | 85% inhibition at 3 mM | Plasmodium falciparum | |
2.7.4.8 | additional information | no inhibition by TMP and by 2-mercaptoethanol up to 5 mM | Plasmodium falciparum | |
2.7.4.8 | UMP | 10-15% inhibition at 5 mM | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Plasmodium falciparum | |
2.7.4.8 | 0.0222 | - |
GMP | wild-type enzyme | Plasmodium falciparum | |
2.7.4.8 | 0.0592 | - |
GMP | mutant Y76F | Plasmodium falciparum | |
2.7.4.8 | 0.0616 | - |
GMP | mutant E101D | Plasmodium falciparum | |
2.7.4.8 | 0.0731 | - |
dGMP | mutant Y76F | Plasmodium falciparum | |
2.7.4.8 | 0.0746 | - |
dGMP | wild-type enzyme | Plasmodium falciparum | |
2.7.4.8 | 0.1816 | - |
dGMP | mutant D74A | Plasmodium falciparum | |
2.7.4.8 | 0.1894 | - |
dGMP | mutant E101D | Plasmodium falciparum | |
2.7.4.8 | 0.1933 | - |
GMP | mutant D74A | Plasmodium falciparum | |
2.7.4.8 | 0.2265 | - |
GMP | mutant N103D | Plasmodium falciparum | |
2.7.4.8 | 0.2364 | - |
dGMP | mutant N103D | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.8 | ATP + GMP | Plasmodium falciparum | - |
ADP + GDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.8 | Plasmodium falciparum | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.8 | 30 | - |
with substrates ATP and dGMP | Plasmodium falciparum |
2.7.4.8 | 750 | - |
with substrate ATP and GMP | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.8 | ATP + AMP | very low activity with AMP | Plasmodium falciparum | ADP + ADP | - |
? | |
2.7.4.8 | ATP + dGMP | low activity, dGMP acts also as inhibitor | Plasmodium falciparum | ADP + dGDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Plasmodium falciparum | ADP + GDP | - |
? | |
2.7.4.8 | additional information | no activity with CMP, dTMP, dAMP, dCMP, and UMP | Plasmodium falciparum | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 2 | 8 | dGMP | mutant Y76F | Plasmodium falciparum | |
2.7.4.8 | 26.5 | - |
dGMP | mutant E101D | Plasmodium falciparum | |
2.7.4.8 | 26.8 | - |
dGMP | mutant N103D | Plasmodium falciparum | |
2.7.4.8 | 33.1 | - |
dGMP | mutant D74A | Plasmodium falciparum | |
2.7.4.8 | 43 | - |
dGMP | wild-type enzyme | Plasmodium falciparum | |
2.7.4.8 | 848 | - |
GMP | mutant E101D | Plasmodium falciparum | |
2.7.4.8 | 946 | - |
GMP | wild-type enzyme | Plasmodium falciparum | |
2.7.4.8 | 1032 | - |
GMP | mutant Y76F | Plasmodium falciparum | |
2.7.4.8 | 1080 | - |
GMP | mutant N103D | Plasmodium falciparum | |
2.7.4.8 | 1121 | - |
GMP | mutant D74A | Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.8 | 7.2 | 7.8 | - |
Plasmodium falciparum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.8 | 6 | 9 | - |
Plasmodium falciparum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 0.148 | - |
dGMP | versus GMP | Plasmodium falciparum | |
2.7.4.8 | 0.4 | - |
dGMP | versus ATP | Plasmodium falciparum |