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Literature summary extracted from

  • Wang, Y.; Zhang, Y.H.
    Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration (2009), Microb. Cell Fact., 8, 30.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima
1.1.1.351 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.01
-
 NADP+ in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima
1.1.1.44 0.011
-
 6-phospho-D-gluconate in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima
1.1.1.44 0.38
-
 NAD+ in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima
1.1.1.351 0.01
-
 NAD+ in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima
1.1.1.351 0.011
-
 6-phospho-D-gluconate with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima
1.1.1.351 0.38
-
 NADP+ in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 53000
-
 SDS-PAGE Thermotoga maritima
1.1.1.351 53000
-
 x * 53000, SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Thermotoga maritima
-
-
-
1.1.1.351 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NAD+
-
 Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+
-
 Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.351 ? x * 53000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Thermotoga maritima
1.1.1.44 6PGDH
-
 Thermotoga maritima
1.1.1.351 6-phosphogluconate dehydrogenase
-
 Thermotoga maritima
1.1.1.351 6PGDH
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.44 90
-
-
 Thermotoga maritima
1.1.1.351 90
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.44 20 95 the enzyme has an approximately 90% of its maximum activity at 80°C, and retains about 2 and about 20% of its maximum activity at 30 and 60°C, respectively Thermotoga maritima
1.1.1.351 30 60 the enzyme retains about 2 and 20% of its maximum activity at 30 and 60°C, respectively Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.44 80 90 the enzyme is highly thermostable in a 50 mM HEPES buffer (pH 6.8) containing 500 mM NaCl, 1 mM EDTA, and 5 mM beta-mercaptoethanol, it retains more than 90% activity for 48 h at 60 and 80°C and retains about 50% enzymatic activity after 48 h at 90°C, the enzyme has half-life times of 48 and 140 h at 90 and 80°C, respectively Thermotoga maritima
1.1.1.351 60 90 the enzyme has half-life times of 48 and 140 h at 90 and 80°C, respectively. The enzyme retains more than 90% activity for 48 h at 60 and 80°C and retains about 50% enzymatic activity after 48 h at 90°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.351 325
-
 6-phospho-D-gluconate with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7
-
-
 Thermotoga maritima
1.1.1.351 7
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.44 6 9 about 70% of 6PGDH activities remain at pH 6.0 and 9.0 Thermotoga maritima
1.1.1.351 6 9 about 70% of enzyme activities remain at pH 6.0 and 9.0 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima
1.1.1.44 NADP+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima
1.1.1.351 NAD+
-
 Thermotoga maritima
1.1.1.351 NADP+ this enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima