Literature summary extracted from
Sims, P.A
Reed, G.H.: Method for the enzymatic synthesis of 2-phospho-D-glycerate from adenosine 5'-triphosphate and D-glycerate via D-glycerate-2-kinase (2005), J. Mol. Catal. B, 32, 77-81.
No PubMed abstract available
Application
EC Number |
Application |
Comment |
Organism |
---|
2.7.1.165 |
synthesis |
synthesis of 2-phospho-D-glycerate, quickly, inexpensively |
Escherichia coli |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.165 |
- |
Escherichia coli |
General Stability
EC Number |
General Stability |
Organism |
---|
2.7.1.165 |
the enzyme does not require dithiothreitol to retain full activity |
Escherichia coli |
2.7.1.165 |
the enzyme retains full activity through at least one freezethaw cycle |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.165 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.1.165 |
- |
Escherichia coli |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.1.165 |
4°C, storage buffer containing 1 mM NaN3, enzyme retains full activity through prolonged storage |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.165 |
ATP + (R)-glycerate |
no activity with (S)-glycerate |
Escherichia coli |
ADP + 2-phospho-(R)-glycerate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.1.165 |
D-glycerate-2-kinase |
- |
Escherichia coli |