Literature summary extracted from

Lacal, J.; Guazzaroni, M.E.; Busch, A.; Krell, T.; Ramos, J.L.
Hierarchical binding of the TodT response regulator to its multiple recognition sites at the tod pathway operon promoter (2008), J. Mol. Biol., 376, 325-337.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.12.11	expression of wild-type TodT, C-terminal part of TodT, and TodT mutants in Escherichia coli strain BL21(DE3). Genes todS and todT are transcribed from a single promoter called PtodX once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates	Pseudomonas putida

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.12.11	additional information	mutations in the different TodT boxes affect TodT binding to the mutant promoters, overview	Pseudomonas putida

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.12.11	additional information	-	additional information	thermodynamic characterization of the molecular interactions between TodT and the C-TodT protein with individual TodT boxes	Pseudomonas putida

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.12.11	28000	-	phosphorylated TodT, gel filtration	Pseudomonas putida
1.14.12.11	108000	-	TodS	Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.12.11	additional information	Pseudomonas putida	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.12.11	Pseudomonas putida	-	genes todS and todT, the expression of the catabolic todXFC1C2BADEGIH operon takes place from a single promoter termed PtodX, located upstream from the todX gene. Its activation is mediated by the TodS/TodT TCS in response to toluene and other aromatic hydrocarbons. The todST genes, located downstream from todH, form an independent operon that is expressed constitutively	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
1.14.12.11	phosphoprotein	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT, TodT phosphorylation occurs only with the folded protein by the TodS sensor kinase, binding site analysis, overview	Pseudomonas putida

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.12.11	additional information	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT	Pseudomonas putida	?	-	?
1.14.12.11	additional information	TodT-P is the active form of the response regulator that induces RNA polymerase to transcribe from the PtodX promoter	Pseudomonas putida	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.12.11	monomer	1 x * 28000, TodT is a monomer in solution	Pseudomonas putida
1.14.12.11	More	in TodT, the C-terminal domain has about 65 amino acids and contains a helix-turn-helix domain	Pseudomonas putida

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.12.11	Tod	-	Pseudomonas putida

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.14.12.11	Pseudomonas putida	about, TodT	-	7.8

General Information

EC Number	General Information	Comment	Organism
1.14.12.11	metabolism	TodS and TodT proteins form a highly specific two-component regulatory system that controls the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway	Pseudomonas putida

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Release 2023.1 (January 2023)