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Literature summary extracted from

  • Gupta, S.D.; Gable, K.; Han, G.; Borovitskaya, A.; Selby, L.; Dunn, T.M.; Harmon, J.M.
    Tsc10p and FVT1: Topologically distinct short-chain reductases required for long-chain base synthesis in yeast and mammals (2009), J. Lipid Res., 50, 1630-1640.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.102 FVT1 expressed in CHO cells or yeast Homo sapiens
1.1.1.102 TSC10p expressed in CHO cells or yeast Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.102 A175T mutation is deleterious to the human enzyme Homo sapiens
1.1.1.102 additional information mutation of any of the three residues that constitute the catalytic triad of FVT1 do not completely inactivate the enzyme, as it is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C. Deletion of residues 4-26 from the FVT1-GFP protein abolishes ER localization Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.102 siRNA reduces expression of transfected FVT1 mRNA and protein by at least 70% Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.102 cytoplasm C-terminus of FVT1 Homo sapiens 5737
-
1.1.1.102 cytoplasm N- and C-terminus of Tsc10p Saccharomyces cerevisiae 5737
-
1.1.1.102 endoplasmic reticulum N-terminal membrane-spanning domain in FVT1 orients to place it in the endoplasmic reticulum lumen Homo sapiens 5783
-
1.1.1.102 endoplasmic reticulum Tsc10p localizes to the yeast endoplasmic reticulum membrane Saccharomyces cerevisiae 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.102 150000
-
 gel filtration Saccharomyces cerevisiae
1.1.1.102 150000
-
 gel filtration1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.102 Homo sapiens
-
-
-
1.1.1.102 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.102 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.102 3-dehydrosphinganine + NADPH + H+
-
 Homo sapiens sphinganine + NADP+
-
 ?
1.1.1.102 3-dehydrosphinganine + NADPH + H+
-
 Saccharomyces cerevisiae sphinganine + NADP+
-
 ?
1.1.1.102 additional information FVT1 can replace Tsc10p in yeast Homo sapiens ?
-
 ?
1.1.1.102 additional information FVT1 is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.102 tetramer gel filtration Homo sapiens
1.1.1.102 tetramer gel filtration Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.102 Fvt1
-
 Homo sapiens
1.1.1.102 Tsc10p
-
 Saccharomyces cerevisiae