EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.8.4 | expression in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.8.4 | H109R | natural polymorphism identified in a dimethylglycine dehydrogenase-deficient patient. Mutant shows only 47% of the wild-type level of bound flavin, a 27fold decrease in specific activity and a 65fold increase in Km value | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.8.4 | 0.039 | - |
N,N-dimethylglycine | and second value 15.4 mM, wild-type, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 0.045 | - |
N,N-dimethylglycine | and second value 1.10 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 1.1 | - |
N,N-dimethylglycine | and first value 0.045 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 2.5 | 5 | N,N-dimethylglycine | mutant H109R, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 15.4 | - |
N,N-dimethylglycine | and first value 0.039 mM, wild-type, pH 7.5, 25°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.8.4 | Homo sapiens | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.8.4 | 0.0068 | - |
mutant H109R, pH 7.5, 25°C | Homo sapiens |
1.5.8.4 | 0.165 | - |
wild-type, pH 7.5, 25°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.8.4 | N,N-dimethylglycine + ferricenium hexafluorophosphate + H2O | - |
Homo sapiens | sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.8.4 | DMGDH | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.8.4 | 0.01 | - |
N,N-dimethylglycine | mutant H109R, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 0.225 | - |
N,N-dimethylglycine | wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
1.5.8.4 | 0.3 | - |
N,N-dimethylglycine | wild-type, pH 7.5, 25°C | Homo sapiens |