Any feedback?
Literature summary extracted from
- Differential in vitro inhibition of polyphenoloxidase from a wild edible mushroom Lactarius salmonicolor (2009), J. Enzyme Inhib. Med. Chem., 24, 464-470.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | sulfosalicylic acid | - |
Lactarius salmonicolor |  |
| 1.14.18.1 | sulfosalicylic acid | - |
Lactarius salmonicolor | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | beta-mercaptoethanol | - |
Lactarius salmonicolor | |
| 1.10.3.1 | glutathione | - |
Lactarius salmonicolor | |
| 1.10.3.1 | L-cysteine | - |
Lactarius salmonicolor | |
| 1.10.3.1 | L-tyrosine | - |
Lactarius salmonicolor | |
| 1.10.3.1 | oxalic acid | - |
Lactarius salmonicolor | |
| 1.10.3.1 | p-aminobenzenesulfonamide | - |
Lactarius salmonicolor | |
| 1.10.3.1 | syringic acid | - |
Lactarius salmonicolor | |
| 1.14.18.1 | beta-mercaptoethanol | uncompetitive inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | glutathione | mixed-type inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | L-cysteine | uncompetitive inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | L-tyrosine | uncompetitive inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | oxalic acid | uncompetitive inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | p-aminobenzenesulfonamide | competitive inhibition | Lactarius salmonicolor | |
| 1.14.18.1 | syringic acid | uncompetitive inhibition | Lactarius salmonicolor | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | 1.809 | - |
4-methylcatechol | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 9.465 | - |
pyrogallol | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 25 | - |
catechol | - |
Lactarius salmonicolor | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 65000 | - |
SDS-PAGE | Lactarius salmonicolor |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.1 | Lactarius salmonicolor | - |
- |
- |
| 1.14.18.1 | Lactarius salmonicolor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.1 | using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column | Lactarius salmonicolor |
| 1.14.18.1 | ammonium sulfate precipitation and Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column chromatography | Lactarius salmonicolor |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
the crude extract has a specific activity of 4654.2 units/mg and the 26.6fold purified enzyme has a specific activity of 123809.5 units/mg, one unit is defined as the amount of enzyme that causes an increase of absorbance of 0.001 unit/min for 1 ml of enzyme at 25°C | Lactarius salmonicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.1 | 2 catechol + O2 | - |
Lactarius salmonicolor | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.10.3.1 | 4-methylcatechol + O2 | - |
Lactarius salmonicolor | ? | - |
? | |
| 1.10.3.1 | pyrogallol + O2 | - |
Lactarius salmonicolor | ? | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | - |
Lactarius salmonicolor | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 4-methylcatechol + O2 | - |
Lactarius salmonicolor | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 | - |
Lactarius salmonicolor | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | pyrogallol + O2 | - |
Lactarius salmonicolor | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.10.3.1 | LsPPO | - |
Lactarius salmonicolor |
| 1.10.3.1 | polyphenoloxidase | - |
Lactarius salmonicolor |
| 1.14.18.1 | polyphenoloxidase | - |
Lactarius salmonicolor |
| 1.14.18.1 | PPO | - |
Lactarius salmonicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 5 | - |
incubation time: 5 min, optimal temperature depends on incubation time | Lactarius salmonicolor |
| 1.10.3.1 | 25 | - |
assay at | Lactarius salmonicolor |
| 1.10.3.1 | 30 | - |
incubation time: 40 min, optimal temperature depends on incubation time | Lactarius salmonicolor |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 5 | 50 | - |
Lactarius salmonicolor |
| 1.14.18.1 | 5 | 50 | at the shortest treatment time (5 min) the highest activity is seen at 5°C | Lactarius salmonicolor |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 30 | 50 | activity is reasonably maintained after 40 min of incubation at temperature below 30°C, significant inactivation becomes apparent at 40°C, and more so at 50°C, enzyme activity decreases due to heat denaturation with increasing temperature | Lactarius salmonicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 6 | - |
substrate: 4-methylcatechol, optimal pH is dependent of the substrate | Lactarius salmonicolor |
| 1.10.3.1 | 7 | - |
substrate: catechol, optimal pH is dependent of the substrate | Lactarius salmonicolor |
| 1.10.3.1 | 7.5 | - |
substrate: pyrogallol 4-methylcatechol, optimal pH is dependent of the substrate | Lactarius salmonicolor |
| 1.14.18.1 | 6 | - |
using 4-methylcatechol as substrate | Lactarius salmonicolor |
| 1.14.18.1 | 7 | - |
using pyrogallol as substrate | Lactarius salmonicolor |
| 1.14.18.1 | 7.5 | - |
using catechol as substrate | Lactarius salmonicolor |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 4.5 | 11 | - |
Lactarius salmonicolor |
| 1.14.18.1 | 4.5 | 11 | - |
Lactarius salmonicolor |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | 0.0000743 | - |
oxalic acid | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 0.00067 | - |
glutathione | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 0.0031 | - |
beta-mercaptoethanol | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 0.0145 | - |
L-cysteine | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 0.0214 | - |
syringic acid | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 0.0267 | - |
p-aminobenzenesulfonamide | - |
Lactarius salmonicolor | |
| 1.10.3.1 | 1.75 | - |
L-tyrosine | - |
Lactarius salmonicolor | |
| 1.14.18.1 | 0.0000743 | - |
oxalic acid | at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.0067 | - |
glutathione | competitive inhibition, at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.0214 | - |
syringic acid | at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.031 | - |
beta-mercaptoethanol | at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.145 | - |
L-cysteine | at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.16 | - |
glutathione | noncompetitive inhibition, at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 0.267 | - |
p-aminobenzenesulfonamide | at 25°C | Lactarius salmonicolor | |
| 1.14.18.1 | 1.75 | - |
L-tyrosine | at 25°C | Lactarius salmonicolor | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | 0.00038 | - |
- |
Lactarius salmonicolor | L-tyrosine | |
| 1.10.3.1 | 0.12 | - |
- |
Lactarius salmonicolor | oxalic acid | |
| 1.10.3.1 | 0.15 | - |
- |
Lactarius salmonicolor | L-cysteine | |
| 1.10.3.1 | 0.23 | - |
- |
Lactarius salmonicolor | p-aminobenzenesulfonamide | |
| 1.10.3.1 | 0.4 | - |
- |
Lactarius salmonicolor | syringic acid | |
| 1.10.3.1 | 0.49 | - |
- |
Lactarius salmonicolor | beta-mercaptoethanol | |
| 1.10.3.1 | 0.91 | - |
- |
Lactarius salmonicolor | glutathione | |
| 1.14.18.1 | 0.00038 | - |
at 25°C | Lactarius salmonicolor | L-tyrosine | |
| 1.14.18.1 | 0.12 | - |
at 25°C | Lactarius salmonicolor | oxalic acid | |
| 1.14.18.1 | 0.15 | - |
at 25°C | Lactarius salmonicolor | L-cysteine | |
| 1.14.18.1 | 0.23 | - |
at 25°C | Lactarius salmonicolor | p-aminobenzenesulfonamide | |
| 1.14.18.1 | 0.4 | - |
at 25°C | Lactarius salmonicolor | syringic acid | |
| 1.14.18.1 | 0.49 | - |
at 25°C | Lactarius salmonicolor | beta-mercaptoethanol | |
| 1.14.18.1 | 0.91 | - |
at 25°C | Lactarius salmonicolor | glutathione | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
