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Literature summary extracted from

  • Musso-Buendia, J.A.; Vidal, A.E.; Kasinthan, G.; Nguyen, C.; Carrero-Lerida, J.; Ruiz-Perez, L.M.; Wilson, K.; Johansson, N.G.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
    Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni (2009), J. Enzyme Inhib. Med. Chem., 24, 111-116.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 drug development the enzyme is a target for drugs against campylobacteriosis Campylobacter jejuni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 overexpression in Escherichia coli strain BL21(DE3) Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 2'-deoxy-5'-phenylmethylaminouracil
-
 Homo sapiens
3.6.1.23 2'-deoxy-5'-triphenylmethanyluracil
-
 Homo sapiens
3.6.1.23 2'-deoxyuracil 5'-diphenyl phosphate
-
 Homo sapiens
3.6.1.23 3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione
-
 Homo sapiens
3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil
-
 Homo sapiens
3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil
-
 Homo sapiens
3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil
-
 Homo sapiens
3.6.1.23 5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil
-
 Homo sapiens
3.6.1.23 5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil
-
 Homo sapiens
3.6.1.23 5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil
-
 Homo sapiens
3.6.1.23 5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil
-
 Homo sapiens
3.6.1.23 alpha,beta-imido-dUDP potent inhibition Campylobacter jejuni
3.6.1.23 dUMP product inhibition Campylobacter jejuni
3.6.1.23 dUMP product inhibition Homo sapiens
3.6.1.23 additional information several dUMP analogues with substituents at the 3'- or 5'-positions show poor inhibitory activity Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 additional information
-
 additional information Michaelis-Menten kinetics, kinetics analysis Campylobacter jejuni
3.6.1.23 0.00066
-
 dUTP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ activates Homo sapiens
3.6.1.23 Mg2+ activates Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Homo sapiens dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Campylobacter jejuni dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Campylobacter jejuni
-
-
-
3.6.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant enzyme from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography, and two steps of anion exchange chromatography Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUDP + H2O the enzyme is highly specific for deoxyuridine nucleotides Homo sapiens deoxyuridine + diphosphate
-
 ?
3.6.1.23 dUDP + H2O the enzyme is highly specific for deoxyuridine nucleotides Campylobacter jejuni deoxyuridine + diphosphate
-
 ?
3.6.1.23 dUTP + H2O dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes Campylobacter jejuni dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is highly specific for deoxyuridine nucleotides Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is highly specific for deoxyuridine nucleotides Campylobacter jejuni dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 dimer
-
 Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Homo sapiens
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Campylobacter jejuni
3.6.1.23 dUTPase
-
 Homo sapiens
3.6.1.23 dUTPase
-
 Campylobacter jejuni
3.6.1.23 dUTPase-dUDPase
-
 Homo sapiens
3.6.1.23 dUTPase-dUDPase
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 25
-
 assay at Homo sapiens
3.6.1.23 25
-
 assay at Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 12.3
-
 dUTP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 8
-
 assay at Homo sapiens
3.6.1.23 8
-
 assay at Campylobacter jejuni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.23 additional information
-
 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
3.6.1.23 additional information
-
 5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
3.6.1.23 additional information
-
 5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
3.6.1.23 additional information
-
 2'-deoxy-5'-phenylmethylaminouracil above 1 mM, pH 8.0, 25°C Homo sapiens
3.6.1.23 0.0114
-
 dUMP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni
3.6.1.23 0.135
-
 2'-deoxyuracil 5'-diphenyl phosphate pH 8.0, 25°C Homo sapiens
3.6.1.23 0.156
-
 3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione pH 8.0, 25°C Homo sapiens
3.6.1.23 0.298
-
 5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
3.6.1.23 0.35
-
 5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
3.6.1.23 0.547
-
 5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C, recombinant enzyme Homo sapiens
3.6.1.23 0.805
-
 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil pH 8.0, 25°C Homo sapiens
3.6.1.23 0.807
-
 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
3.6.1.23 0.908
-
 2'-deoxy-5'-triphenylmethanyluracil pH 8.0, 25°C Homo sapiens