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Literature summary extracted from

  • Nakamura, T.; Yamaguchi, A.; Kondo, H.; Watanabe, H.; Kurihara, T.; Esaki, N.; Hirono, S.; Tanaka, S.
    Roles of K151 and D180 in L-2-haloacid dehalogenase from Pseudomonas sp. YL: Analysis by molecular dynamics and ab initio fragment molecular orbital calculations (2009), J. Comput. Chem., 30, 2625-2634.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.10 D180A the mutation destabilizes the rotation of the nucleophile D10, fixes catalytic water around D10, and prevents K151 from approaching D10 Pseudomonas sp.
3.8.1.10 K151A the mutant is almost inactive, the mutation destabilizes substrate orientation and affects the balance of the charge around the active site Pseudomonas sp.
3.8.1.10 K151R the mutant is almost inactive Pseudomonas sp.
3.8.1.10 S118A the mutant retains 30% of the activity of the wild type enzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.10 L-2-chloropropionate + H2O Pseudomonas sp.
-
 D-lactate + HCl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.10 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.10 L-2-haloacid dehalogenase
-
 Pseudomonas sp.
3.8.1.10 L-DEX YL
-
 Pseudomonas sp.