EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.248 | overexpression in Escherichia coli SG13009 | Papaver somniferum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.248 | D107A | increase in Km, 2fold decrease in kcat | Papaver somniferum |
1.1.1.248 | F104A | increase in kcat | Papaver somniferum |
1.1.1.248 | F104A/I275A | shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type | Papaver somniferum |
1.1.1.248 | I275A | increase in Km, shows an increase of velocity by a factor of 2.3 | Papaver somniferum |
1.1.1.248 | K186V | moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type | Papaver somniferum |
1.1.1.248 | L185A | increase in Km, decrease in kcat | Papaver somniferum |
1.1.1.248 | L266A | decrease in kcat | Papaver somniferum |
1.1.1.248 | M271A | decrease in kcat | Papaver somniferum |
1.1.1.248 | M271A | increase in Km | Papaver somniferum |
1.1.1.248 | N272A | increase in Km, decrease in kcat | Papaver somniferum |
1.1.1.248 | S181A | moderate effects on substrate affinity, increase in kcat by 50% | Papaver somniferum |
1.1.1.248 | T182A | moderate effects on substrate affinity | Papaver somniferum |
1.1.1.248 | V106A | moderate effects on substrate affinity, increase in kcat by 90% | Papaver somniferum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.248 | additional information | strong substrate inhibition of SalR | Papaver somniferum | |
1.1.1.248 | NADP+ | product inhibition | Papaver somniferum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.248 | 0.0021 | - |
salutaridine | wild-type | Papaver somniferum | |
1.1.1.248 | 0.0034 | - |
NADPH | mutant K186V | Papaver somniferum | |
1.1.1.248 | 0.0035 | - |
NADPH | wild-type | Papaver somniferum | |
1.1.1.248 | 0.0041 | - |
salutaridine | mutant T182A | Papaver somniferum | |
1.1.1.248 | 0.0045 | - |
NADPH | mutant S181A | Papaver somniferum | |
1.1.1.248 | 0.005 | - |
salutaridine | mutant S181A | Papaver somniferum | |
1.1.1.248 | 0.007 | - |
NADPH | mutant V106A | Papaver somniferum | |
1.1.1.248 | 0.0078 | - |
salutaridine | mutant K186V | Papaver somniferum | |
1.1.1.248 | 0.0089 | - |
salutaridine | mutant V106A | Papaver somniferum | |
1.1.1.248 | 0.0097 | - |
NADPH | mutant F104A | Papaver somniferum | |
1.1.1.248 | 0.0132 | - |
salutaridine | mutant L185A | Papaver somniferum | |
1.1.1.248 | 0.0148 | - |
salutaridine | mutant D107A | Papaver somniferum | |
1.1.1.248 | 0.0155 | - |
NADPH | mutant L266A | Papaver somniferum | |
1.1.1.248 | 0.0156 | - |
NADPH | mutant N272A | Papaver somniferum | |
1.1.1.248 | 0.0198 | - |
NADPH | mutant M271A | Papaver somniferum | |
1.1.1.248 | 0.0211 | - |
NADPH | mutant I275A | Papaver somniferum | |
1.1.1.248 | 0.0213 | - |
NADPH | mutant T182A | Papaver somniferum | |
1.1.1.248 | 0.0225 | - |
salutaridine | mutant M271A | Papaver somniferum | |
1.1.1.248 | 0.0297 | - |
NADPH | mutant L185A | Papaver somniferum | |
1.1.1.248 | 0.0305 | - |
NADPH | mutant D107A | Papaver somniferum | |
1.1.1.248 | 0.0318 | - |
salutaridine | mutant F104A | Papaver somniferum | |
1.1.1.248 | 0.0331 | - |
salutaridine | mutant I275A | Papaver somniferum | |
1.1.1.248 | 0.043 | - |
NADPH | mutant F104A/I275A | Papaver somniferum | |
1.1.1.248 | 0.0494 | - |
salutaridine | mutant L266A | Papaver somniferum | |
1.1.1.248 | 0.0901 | - |
salutaridine | mutant N272A | Papaver somniferum | |
1.1.1.248 | 0.4086 | - |
salutaridine | mutant F104A/I275A | Papaver somniferum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.248 | Papaver somniferum | Q071N0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.248 | to homogeneity | Papaver somniferum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.248 | salutaridine + NADPH + H+ | - |
Papaver somniferum | (7S)-salutaridinol + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.248 | SalR | - |
Papaver somniferum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.248 | 0.006 | - |
salutaridine | mutant M271A | Papaver somniferum | |
1.1.1.248 | 0.016 | - |
salutaridine | mutant L185A | Papaver somniferum | |
1.1.1.248 | 0.021 | - |
salutaridine | mutant N272A | Papaver somniferum | |
1.1.1.248 | 0.126 | - |
salutaridine | mutant T182A | Papaver somniferum | |
1.1.1.248 | 0.501 | - |
salutaridine | mutant L266A | Papaver somniferum | |
1.1.1.248 | 0.61 | - |
salutaridine | mutant D107A | Papaver somniferum | |
1.1.1.248 | 0.998 | - |
salutaridine | mutant K186V | Papaver somniferum | |
1.1.1.248 | 1.306 | - |
salutaridine | wild-type | Papaver somniferum | |
1.1.1.248 | 1.933 | - |
salutaridine | mutant S181A | Papaver somniferum | |
1.1.1.248 | 2.45 | - |
salutaridine | mutant F104A/I275A | Papaver somniferum | |
1.1.1.248 | 2.458 | - |
salutaridine | mutant V106A | Papaver somniferum | |
1.1.1.248 | 2.727 | - |
salutaridine | mutant F104A | Papaver somniferum | |
1.1.1.248 | 3.188 | - |
salutaridine | mutant I275A | Papaver somniferum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.248 | NADPH | - |
Papaver somniferum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.248 | 0.0012 | - |
NADP+ | - |
Papaver somniferum |