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Literature summary extracted from

  • Quaye, O.; Cowins, S.; Gadda, G.
    Contribution of flavin covalent linkage with histidine 99 to the reaction catalyzed by choline oxidase (2009), J. Biol. Chem., 284, 16990-16997.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 H99N mutant, analysis of kinetic parameters Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 additional information
-
 additional information analysis of substrate kinetic isotope effects Arthrobacter globiformis
1.1.3.17 1.3
-
 choline substrate choline, pH 7, 25°C Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis Q7X2H8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.17 purified with the bound flavin cofactor in the fully oxidized state Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.17 0.08
-
 H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis
1.1.3.17 0.28
-
 apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 choline oxidase
-
 Arthrobacter globiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.17 additional information
-
 analysis of temperature effect on rate constant for flavin reduction Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.17 1.03
-
 choline apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis
1.1.3.17 1.86
-
 choline H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 8 11 H99N mutant Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.17 6 8 H99N mutant, lower KM compared to pH 8-11. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis
1.1.3.17 8 11 H99N mutant, no impact on KM, highest KM. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD
-
 Arthrobacter globiformis