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Literature summary extracted from

  • Ordonez, E.; Van Belle, K.; Roos, G.; De Galan, S.; Letek, M.; Gil, J.A.; Wyns, L.; Mateos, L.M.; Messens, J.
    Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange (2009), J. Biol. Chem., 284, 15107-15116.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.3 expression in Escherichia coli Corynebacterium glutamicum
2.8.4.2 expression in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.8.4.2 additional information gene deletion mutants of isoforms ArsC1 and ArsC2 show similar resistance levels to As(V) as wild-type. the ArsC1/ArsC2 double mutant strain is very sensitive to arsenate and shows resistance levels, which are 20–30 times lower. Only plasmids containing either the arsC1 or the arsC2 gene are able to complement the arsenate reductase activity. When the single cysteine in the arsC1 and the arsC2 genes is mutated to a serine, the complementation is lost. Removing arsC1, arsC2, or both genes had no effect on the resistance against As(III), and resistance levels equivalent to those for wild type are obtained Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.2 0.036
-
arsenate isoform ArsC2, pH 8.0, presence of phosphate Corynebacterium glutamicum
2.8.4.2 0.038
-
arsenate isoform ArsC2, pH 8.0, presence of sulfate Corynebacterium glutamicum
2.8.4.2 0.046
-
arsenate isoform ArsC1, pH 8.0, presence of sulfate Corynebacterium glutamicum
2.8.4.2 0.082
-
arsenate isoform ArsC2, pH 8.0 Corynebacterium glutamicum
2.8.4.2 0.13
-
arsenate isoform ArsC1, pH 8.0, presence of phosphate Corynebacterium glutamicum
2.8.4.2 0.142
-
arsenate isoform ArsC1, pH 8.0 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.4.3 arseno-mycothiol + mycoredoxin Corynebacterium glutamicum reduction of arsenate is part of a defense mechanism of the cell against toxic arsenate. The substrate arseno-mycothiol is formed by arsenate-mycothiol transferase. A second mycothiol recycles mycoredoxin and forms mycothione arsenite + mycothiol-mycoredoxin disulfide
-
?
2.8.4.2 arsenate + mycothiol Corynebacterium glutamicum arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione arseno-mycothiol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.15 Corynebacterium glutamicum
-
-
-
1.20.4.3 Corynebacterium glutamicum
-
-
-
2.8.4.2 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.4.3
-
Corynebacterium glutamicum
2.8.4.2 ArsC1 and ArsC2 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.15 mycothione + NADPH + H+
-
Corynebacterium glutamicum mycothiol + NADP+
-
?
1.20.4.3 arseno-mycothiol + mycoredoxin
-
Corynebacterium glutamicum arsenite + mycothiol-mycoredoxin disulfide a second mycothiol recycles mycoredoxin and forms mycothione ?
1.20.4.3 arseno-mycothiol + mycoredoxin reduction of arsenate is part of a defense mechanism of the cell against toxic arsenate. The substrate arseno-mycothiol is formed by arsenate-mycothiol transferase. A second mycothiol recycles mycoredoxin and forms mycothione Corynebacterium glutamicum arsenite + mycothiol-mycoredoxin disulfide
-
?
2.8.4.2 arsenate + mycothiol arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione Corynebacterium glutamicum arseno-mycothiol
-
?
2.8.4.2 arsenate + mycothiol reduction of arsenate is part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione Corynebacterium glutamicum arseno-mycothiol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.15 MTR
-
Corynebacterium glutamicum
1.8.1.15 mycothione reductase
-
Corynebacterium glutamicum
1.20.4.3 Mrx1
-
Corynebacterium glutamicum
1.20.4.3 MrxI
-
Corynebacterium glutamicum
2.8.4.2 Cg_ArsC1
-
Corynebacterium glutamicum
2.8.4.2 Cg_ArsC2
-
Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.15 37
-
assay at Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.4.2 0.12
-
arsenate isoform ArsC2, pH 8.0, presence of sulfate Corynebacterium glutamicum
2.8.4.2 0.17
-
arsenate isoform ArsC2, pH 8.0, presence of phosphate Corynebacterium glutamicum
2.8.4.2 0.23
-
arsenate isoform ArsC1, pH 8.0, presence of sulfate Corynebacterium glutamicum
2.8.4.2 0.28
-
arsenate isoform ArsC2, pH 8.0 Corynebacterium glutamicum
2.8.4.2 0.53
-
arsenate isoform ArsC1, pH 8.0 Corynebacterium glutamicum
2.8.4.2 0.6
-
arsenate isoform ArsC1, pH 8.0, presence of phosphate Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.15 8
-
assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.15 NADPH
-
Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.8.1.15 malfunction in vivo Mtr knockout strain shows that the enzyme is involved in arsenate (As(V)) resistance suggesting a possible relation with the arsenate reductase activities Corynebacterium glutamicum