Literature summary extracted from
Thorn, A.; Egerer-Sieber, C.; Jaeger, C.; Herl, V.; Mueller-Uri, F.; Kreis, W.; Muller, Y.
The crystal structure of progesterone 5beta-reductase from Digitalis lanata defines a novel class of short chain dehydrogenases/reductases (2008), J. Biol. Chem., 283, 17260-17269.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.3.1.3 |
expressed in Escherichia coli strain M15 |
Digitalis lanata |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.3.1.3 |
5beta-POR, in the presence and absence of the cofactor NADP+, hanging drop vapour diffusion method, using 15% polyethylene glycol 4000, 0.1 M ammonium acetate, and 0.1 M sodium citrate, pH 5.6 |
Digitalis lanata |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.3.1.3 |
Y179A |
inactive |
Digitalis lanata |
1.3.1.3 |
Y179F |
inactive |
Digitalis lanata |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.3.1.3 |
0.12 |
- |
progesterone |
purified wild type enzyme |
Digitalis lanata |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.3.1.3 |
Digitalis lanata |
Q6PQJ9 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.3.1.3 |
nickel-Sepharose affinity column chromatography and Superdex 75 gel filtration |
Digitalis lanata |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.3.1.3 |
1.89 |
- |
purified wild type enzyme, using progesterone as substrate |
Digitalis lanata |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.3.1.3 |
progesterone + NADPH + H+ |
- |
Digitalis lanata |
5beta-pregnane-3,20-dione + NADP+ |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.3.1.3 |
5beta-POR |
- |
Digitalis lanata |
1.3.1.3 |
progesterone 5beta-reductase |
- |
Digitalis lanata |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.3.1.3 |
NADPH |
- |
Digitalis lanata |
|