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Literature summary extracted from

  • Vetting, M.W.; Frantom, P.A.; Blanchard, J.S.
    Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis (2008), J. Biol. Chem., 283, 15834-15844.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.250
-
 Corynebacterium glutamicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.250 vapor diffusion under silicon oil. The structure of MshA is determined both in the absence of substrates and in a complex with UDP and 11D-myo-inositol 3-phosphate. The structure of unliganded CgMshA (APO form) is determined by single isomorphous replacement with anomalous scattering utilizing a CuKalpha home source and a mercury derivative to 2.1 A resolution. Crystallization of CgMshA in the presence of UDP or UDP-GlcNAc results in a tetragonal crystal form. The structure of MshA from Corynebacterium glutamicum is determined both in the absence of substrates and in a complex with UDP and 1L-myo-inositol-1-phosphate Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.250 0.21
-
 UDP-N-acetyl-D-glucosamine pH 7.8, 25°C Corynebacterium glutamicum
2.4.1.250 0.24
-
 1D-myo-inositol 3-phosphate pH 7.8, 25°C Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate Corynebacterium glutamicum 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, committed step of mycothiol biosynthesis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.250 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.250
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, committed step of mycothiol biosynthesis Corynebacterium glutamicum 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1D-myo-inositol 3-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond Corynebacterium glutamicum 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.250 CgMshA
-
 Corynebacterium glutamicum