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Literature summary extracted from
- Functional characterization of Escherichia coli MsbA: interaction with nucleotides and substrates (2008), J. Biol. Chem., 283, 12840-12850.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.5.2.6 | Lipid | from Escherichia coli, about 1.4fold stimulation | Escherichia coli |  |
| 7.5.2.6 | lipid A | about 2fold stimulation of the ATPase activity of MsbA by its substrate | Escherichia coli | |
| 7.5.2.6 | phosphocholine | 2fold stimulation of MsbA | Escherichia coli | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.5.2.6 | gene msbA, overexpression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.5.2.6 | AMP-PCP | - |
Escherichia coli | |
| 7.5.2.6 | D-20133 | lipid-based drug, high affinity binding to MsbA | Escherichia coli | |
| 7.5.2.6 | ilmofosine | lipid-based drug, high affinity binding to MsbA | Escherichia coli | |
| 7.5.2.6 | leupeptin | - |
Escherichia coli | |
| 7.5.2.6 | lipopolysaccharide | dependent on the origin | Escherichia coli | |
| 7.5.2.6 | additional information | no inhibition by substrate lipid A also at high concentration, poor inhibition by verapamil, colchicine, and daunorubicine, poor inhibition by lipid-based drugs D-20133, and D-21266, along with LY335979 | Escherichia coli | |
| 7.5.2.6 | vanadate | - |
Escherichia coli | |
| 7.5.2.6 | vinblastin | - |
Escherichia coli | |
| 7.5.2.6 | vinblastine | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.5.2.6 | additional information | - |
additional information | lipid binding kinetics, overview | Escherichia coli | |
| 7.5.2.6 | 0.0064 | - |
lipid A | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 7.5.2.6 | 0.048 | 0.05 | lipopolysaccharide | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 7.5.2.6 | 2 | - |
ATP | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.5.2.6 | inner membrane | enzyme structure with 6 transmembrane helices, overview | Escherichia coli | - |
- |
| 7.5.2.6 | periplasm | - |
Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.5.2.6 | ATP + H2O + Lipid A/in | Escherichia coli | - |
ADP + phosphate + Lipid A/out | - |
? | |
| 7.5.2.6 | additional information | Escherichia coli | MsbA functions as an ATP-dependent lipid translocase that transports lipid A from the inner to the outer leaflet of the cytoplasmic membrane. MsbA is able to hydrolyze TNP-ATP, albeit at a lower rate than its corresponding ATPase activity | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.5.2.6 | Escherichia coli | - |
gene msbA | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.5.2.6 | ATP + H2O + Lipid A/in | - |
Escherichia coli | ADP + phosphate + Lipid A/out | - |
? | |
| 7.5.2.6 | ATP + H2O + Lipid A/in | substrate binding structure, overview | Escherichia coli | ADP + phosphate + Lipid A/out | - |
? | |
| 7.5.2.6 | additional information | MsbA functions as an ATP-dependent lipid translocase that transports lipid A from the inner to the outer leaflet of the cytoplasmic membrane. MsbA is able to hydrolyze TNP-ATP, albeit at a lower rate than its corresponding ATPase activity | Escherichia coli | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.5.2.6 | homodimer | enzyme structure, modelling | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.5.2.6 | More | the enzyme belongs to the ATP-binding cassette superfamily | Escherichia coli |
| 7.5.2.6 | MsbA | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.5.2.6 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.5.2.6 | 7.5 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.5.2.6 | ATP | dependent on | Escherichia coli | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 7.5.2.6 | 0.48 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | leupeptin | |
| 7.5.2.6 | 0.5 | - |
above, pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | vinblastin | |
| 7.5.2.6 | 1.25 | - |
pH 7.5, 37°C, recombinant His-tagged protein | Escherichia coli | AMP-PCP | |
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Release 2023.1 (January 2023)
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