EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.87 | overexpressed using a T7lac promoter-driven construct, hexahistidine-tagged fusion protein | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.87 | cytoplasm | - |
Escherichia coli | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.87 | UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | Escherichia coli | ArnB catalyzes the reversible transfer of the amino group from glutamate to the acceptor, uridine 5'-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate), the intermediate that is synthesized by ArnA from UDP-glucuronic acid.the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.87 | Escherichia coli | P77690 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.87 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.87 | UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | ArnB catalyzes the reversible transfer of the amino group from glutamate to the acceptor, uridine 5'-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate), the intermediate that is synthesized by ArnA from UDP-glucuronic acid.the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable | Escherichia coli | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r | |
2.6.1.87 | UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable. The rate of transamination with L-methionine, L-glutamine, and L-alanine is measurable at 5%, 2%, and 1%, respectively, of the rate observed with L-glutamate | Escherichia coli | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.87 | 30 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.87 | 7.5 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.87 | pyridoxal 5'-phosphate | the pyridoxal phosphate is converted to the pyridoxamine form in the presence of excess glutamate | Escherichia coli |