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Literature summary extracted from

  • Yeom, J.; Jeon, C.O.; Madsen, E.L.; Park, W.
    In vitro and in vivo interactions of ferredoxin-NADP+ reductases in Pseudomonas putida (2009), J. Biochem., 145, 481-491.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed via plasmid in Escherichia coli strain BL21(DE3) Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.00083
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 0.00111
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 0.00112
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 0.00181
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 0.00191
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 0.00191
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 0.00245
-
 NADPH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 0.00263
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 0.00298
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 0.00326
-
 NADPH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 0.00397
-
 NADPH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 0.00464
-
 NADH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 0.00479
-
 NADPH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 0.00615
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 0.00686
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 0.00717
-
 NADH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 0.00802
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 0.00822
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 0.00933
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 0.00943
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 0.01063
-
 NADH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 0.01163
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 0.02363
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 0.0632
-
 NADH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 27000
-
 SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 [4Fe-4S]-ferredoxin + NADPH Pseudomonas putida [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) reduced [4Fe-4S]-ferredoxin + NADP+
-
 r
1.18.1.2 [4Fe-4S]-ferredoxin + NADPH Pseudomonas putida KT 2240 [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) reduced [4Fe-4S]-ferredoxin + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Pseudomonas putida
-
 wild type and mutants fprA (lacking FprA) and fprB (lacking FprB)
-
1.18.1.2 Pseudomonas putida KT 2240
-
 wild type and mutants fprA (lacking FprA) and fprB (lacking FprB)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADH + K3Fe(CN)6
-
 Pseudomonas putida NAD+ + reduced K3Fe(CN)6
-
 r
1.18.1.2 NADH + K3Fe(CN)6
-
 Pseudomonas putida KT 2240 NAD+ + reduced K3Fe(CN)6
-
 r
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida KT 2240 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.18.1.2 NADPH + K3Fe(CN)6 assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida NADP+ + reduced K3Fe(CN)6
-
 r
1.18.1.2 NADPH + K3Fe(CN)6 assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida KT 2240 NADP+ + reduced K3Fe(CN)6
-
 r
1.18.1.2 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Pseudomonas putida NAD+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.18.1.2 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Pseudomonas putida KT 2240 NAD+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.18.1.2 oxidized ferredoxin + NADH + cytochrome c
-
 Pseudomonas putida reduced ferredoxin + NAD+ + reduced cytochrome c
-
 r
1.18.1.2 oxidized ferredoxin + NADPH + cytochrome c assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida reduced ferredoxin + NADP+ + reduced cytochrome c
-
 r
1.18.1.2 [4Fe-4S]-ferredoxin + NADPH [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) Pseudomonas putida reduced [4Fe-4S]-ferredoxin + NADP+
-
 r
1.18.1.2 [4Fe-4S]-ferredoxin + NADPH [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) Pseudomonas putida KT 2240 reduced [4Fe-4S]-ferredoxin + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 27000, fast protein liquid chromatography Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
 Pseudomonas putida
1.18.1.2 FPR
-
 Pseudomonas putida
1.18.1.2 FprA Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. Pseudomonas putida
1.18.1.2 FprB Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 1.16
-
 NADH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 3.03
-
 NADH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 3.09
-
 NADH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 3.82
-
 NADPH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 4.51
-
 NADPH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 4.59
-
 NADH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 4.77
-
 NADPH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 5.99
-
 NADPH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 70
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 90
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 90
-
 NADH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 110
-
 NADH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 130
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 170
-
 NADH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 530
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 540
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 680
-
 NADH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 770
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor Pseudomonas putida
1.18.1.2 860
-
 NADH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 1000
-
 NADPH for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 1130
-
 NADPH for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 1140
-
 NADPH for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor Pseudomonas putida
1.18.1.2 1370
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 1860
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 3050
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 3230
-
 NADPH for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor Pseudomonas putida
1.18.1.2 3720
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida
1.18.1.2 4590
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 4630
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor Pseudomonas putida
1.18.1.2 6730
-
 NADH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 11000
-
 NADPH for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor Pseudomonas putida
1.18.1.2 11510
-
 NADPH for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor Pseudomonas putida