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Literature summary extracted from

  • Ishimizu, T.; Sano, K.; Uchida, T.; Teshima, H.; Omichi, K.; Hojo, H.; Nakahara, Y.; Hase, S.
    Purification and substrate specificity of UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase involved in the biosynthesis of the Xylalpha1-3Xylalpha1-3Glcbeta1-O-Ser on epidermal growth factor-like domains (2007), J. Biochem., 141, 593-600.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.42 Co2+ 20 mM, 85% loss of activity Bos taurus
2.4.2.42 Cu2+ 20 mM, complete loss of activity Bos taurus
2.4.2.42 EDTA
-
 Bos taurus
2.4.2.42 Fe2+ 20 mM, complete loss of activity Bos taurus
2.4.2.42 Ni2+ 20 mM, 90% loss of activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.42 0.034
-
 Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) pH 7.2, 37°C Bos taurus
2.4.2.42 4.2
-
 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside pH 7.2, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.42 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.42 Mn2+ 20 mM, 2fold increase in activity Bos taurus
2.4.2.42 additional information 20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.42 UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) Bos taurus the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.42 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.42
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.42 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.42 0.075
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.42 additional information no activity with UDP-D-glucose Bos taurus ?
-
 ?
2.4.2.42 UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
-
 Bos taurus UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
-
 ?
2.4.2.42 UDP-D-xylose + D-glucose
-
 Bos taurus UDP + Xylalpha(1-3)Glc
-
 ?
2.4.2.42 UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains Bos taurus UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
 ?
2.4.2.42 UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide Bos taurus UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.42 UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.42 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.42 7
-
 around Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.42 6 8 pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity Bos taurus