BRENDA - Enzyme Database

Purification and substrate specificity of UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase involved in the biosynthesis of the Xylalpha1-3Xylalpha1-3Glcbeta1-O-Ser on epidermal growth factor-like domains

Ishimizu, T.; Sano, K.; Uchida, T.; Teshima, H.; Omichi, K.; Hojo, H.; Nakahara, Y.; Hase, S.; J. Biochem. 141, 593-600 (2007)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.42
Co2+
20 mM, 85% loss of activity
Bos taurus
2.4.2.42
Cu2+
20 mM, complete loss of activity
Bos taurus
2.4.2.42
EDTA
-
Bos taurus
2.4.2.42
Fe2+
20 mM, complete loss of activity
Bos taurus
2.4.2.42
Ni2+
20 mM, 90% loss of activity
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.42
0.034
-
Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
pH 7.2, 37°C
Bos taurus
2.4.2.42
4.2
-
2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
pH 7.2, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.42
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.42
Mn2+
20 mM, 2fold increase in activity
Bos taurus
2.4.2.42
additional information
20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
Bos taurus
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.42
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.42
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.42
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.42
0.075
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.42
additional information
no activity with UDP-D-glucose
698648
Bos taurus
?
-
-
-
-
2.4.2.42
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
-
698648
Bos taurus
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
-
-
-
?
2.4.2.42
UDP-D-xylose + D-glucose
-
698648
Bos taurus
UDP + Xylalpha(1-3)Glc
-
-
-
?
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.42
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.42
7
-
around
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.42
6
8
pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.42
Co2+
20 mM, 85% loss of activity
Bos taurus
2.4.2.42
Cu2+
20 mM, complete loss of activity
Bos taurus
2.4.2.42
EDTA
-
Bos taurus
2.4.2.42
Fe2+
20 mM, complete loss of activity
Bos taurus
2.4.2.42
Ni2+
20 mM, 90% loss of activity
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.42
0.034
-
Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
pH 7.2, 37°C
Bos taurus
2.4.2.42
4.2
-
2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
pH 7.2, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.42
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.42
Mn2+
20 mM, 2fold increase in activity
Bos taurus
2.4.2.42
additional information
20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
Bos taurus
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.42
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.42
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.42
0.075
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.42
additional information
no activity with UDP-D-glucose
698648
Bos taurus
?
-
-
-
-
2.4.2.42
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
-
698648
Bos taurus
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
-
-
-
?
2.4.2.42
UDP-D-xylose + D-glucose
-
698648
Bos taurus
UDP + Xylalpha(1-3)Glc
-
-
-
?
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
2.4.2.42
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.42
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.42
7
-
around
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.42
6
8
pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity
Bos taurus