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Literature summary extracted from

  • Ohmoto, T.; Kinoshita, T.; Moriyoshi, K.; Sakai, K.; Hamada, N.; Ohe, T.
    Purification and some properties of 2-hydroxychromene-2-carboxylate isomerase from naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 (1998), J. Biochem., 124, 591-597.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.99.1.4 glutathione activates above 0.15 mM Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.99.1.4 Cu2+
-
 Pseudomonas sp.
5.99.1.4 Hg2+
-
 Pseudomonas sp.
5.99.1.4 iodoacetic acid
-
 Pseudomonas sp.
5.99.1.4 monoiodoacetate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.99.1.4 0.053
-
 2-hydroxy-2H-chromene-2-carboxylate pH 7.4, 30°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.99.1.4 25000
-
 1 * 25000, SDS-PAGE Pseudomonas sp.
5.99.1.4 27000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
5.99.1.4 Pseudomonas sp.
-
-
-
5.99.1.4 Pseudomonas sp. TA-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.99.1.4
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.99.1.4 62
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.99.1.4 2-hydroxy-2H-chromene-2-carboxylate the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum Pseudomonas sp. (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate ?
5.99.1.4 2-hydroxy-2H-chromene-2-carboxylate the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum Pseudomonas sp. TA-2 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate ?

Subunits

EC Number Subunits Comment Organism
5.99.1.4 monomer 1 * 25000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
5.99.1.4 2HC2CA isomerase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.99.1.4 8
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.99.1.4 6.8 9.5 pH 6.8: about 35% of maximal activity, pH 9.5: about 40% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.99.1.4 45
-
 10 min, stable at temperature up to Pseudomonas sp.
5.99.1.4 50
-
 10 min, in presence of glutathione at 2.5 mM, the enzyme is stable up to Pseudomonas sp.
5.99.1.4 55
-
 10 min, 13% of the original activity remains. In presence of glutathione at 2.5 mM, 68% of the original activity remains after 10 min Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.99.1.4 1.5 10 4°C, 40 h, stable in the range of pH 1.5 to 10.0 Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.99.1.4 Pseudomonas sp. isoelectric focusing
-
 5