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Literature summary extracted from

  • Kuhm, A.E.; Stolz, A.; Ngai, K.L.; Knackmuss, H.J.
    Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids (1991), J. Bacteriol., 173, 3795-3802.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.56 enzyme in crude extract is stable in Na-K phosphate buffer, pH 7.5, after 1 week of storage (2.4 mg of protein per ml) at 4°C, 100% of the original activity is recovered Sphingobium xenophagum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.56 0.034
-
1,2-dihydroxynaphthalene
-
Sphingobium xenophagum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.56 Fe2+ required for maximal activity Sphingobium xenophagum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.56 33000
-
8 * 33000, SDS-PAGE Sphingobium xenophagum
1.13.11.56 290000
-
gel filtration Sphingobium xenophagum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.56 1,2-dihydroxynaphthalene + O2 Sphingobium xenophagum induced in presence of naphthalene-2-sulfonic acid 2-hydroxychromene-2-carboxylate
-
?
1.13.11.56 1,2-dihydroxynaphthalene + O2 Sphingobium xenophagum BN6 induced in presence of naphthalene-2-sulfonic acid 2-hydroxychromene-2-carboxylate
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.56 Acetone storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated Sphingobium xenophagum
1.13.11.56 Ethanol storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated Sphingobium xenophagum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.56 Sphingobium xenophagum
-
BN6
-
1.13.11.56 Sphingobium xenophagum BN6
-
BN6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.56
-
Sphingobium xenophagum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.56 83.36
-
-
Sphingobium xenophagum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.56 1,2,5-trihydroxynaphthalene + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 1,2,6-trihydroxynaphthalene + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 1,2,7-trihydroxynaphthalene + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 1,2-dihydroxynaphthalene + O2 induced in presence of naphthalene-2-sulfonic acid Sphingobium xenophagum 2-hydroxychromene-2-carboxylate
-
?
1.13.11.56 1,2-dihydroxynaphthalene + O2 induced in presence of naphthalene-2-sulfonic acid Sphingobium xenophagum BN6 2-hydroxychromene-2-carboxylate
-
?
1.13.11.56 1,2-dihydroxynaphthalene + O2
-
Sphingobium xenophagum 2-hydroxy-2H-chromene-2-carboxylate
-
?
1.13.11.56 2,3-dihydroxybiphenyl + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 2,3-dihydroxybiphenyl + O2
-
Sphingobium xenophagum BN6 ?
-
?
1.13.11.56 3,4-dihydroxybiphenyl + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 3-methylcatechol + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 3-methylcatechol + O2
-
Sphingobium xenophagum BN6 ?
-
?
1.13.11.56 4-methylcatechol + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 4-methylcatechol + O2
-
Sphingobium xenophagum BN6 ?
-
?
1.13.11.56 catechol + O2
-
Sphingobium xenophagum ?
-
?
1.13.11.56 catechol + O2
-
Sphingobium xenophagum BN6 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.56 octamer 8 * 33000, SDS-PAGE Sphingobium xenophagum

Synonyms

EC Number Synonyms Comment Organism
1.13.11.56 1,2-DHN dioxygenase
-
Sphingobium xenophagum
1.13.11.56 DHNDO
-
Sphingobium xenophagum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.56 additional information
-
the enzyme is more stable at slightly alkaline pH values and better preserved in Na-K phosphate buffer than in Tris-HCI or glycine-NaOH Sphingobium xenophagum