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Literature summary extracted from

  • Patel, T.R.; Barnsley, E.A.
    Naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase (1980), J. Bacteriol., 143, 668-673.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.56 the enzyme is inactivated slowly on standing, and inactivation is accelerated by dilution with aerated buffers and by H2O2. The inactive enzyme is reactivated by anaerobic incubation with Fe(SO4) and ferrous ammonium sulfate. Thiol reagents and acetone, ethanol, or glycerol decrease the rate of loss of activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.56 1,2,3-trihydroxybenzene 0.0005 mM, 76% inhibition Pseudomonas putida
1.13.11.56 2,2'-dipyridyl
-
 Pseudomonas putida
1.13.11.56 2,3-dihydroxynaphthalene 0.0005 mM, 44% inhibition Pseudomonas putida
1.13.11.56 2,7-dihydroxynaphthalene 0.0005 mM, 64% inhibition Pseudomonas putida
1.13.11.56 3,4-dihydroxybenzoic acid 0.0005 mM, 20% inhibition Pseudomonas putida
1.13.11.56 3-methylcatechol 0.0005 mM, 76% inhibition, competitive inhibitor Pseudomonas putida
1.13.11.56 4-methylcatechol 0.0005 mM, 36% inhibition, competitive inhibitor Pseudomonas putida
1.13.11.56 8-hydroxyquinoline
-
 Pseudomonas putida
1.13.11.56 bathophenanthroline sulfonate
-
 Pseudomonas putida
1.13.11.56 Co2+
-
 Pseudomonas putida
1.13.11.56 Cu2+
-
 Pseudomonas putida
1.13.11.56 Fe3+
-
 Pseudomonas putida
1.13.11.56 Hg2+
-
 Pseudomonas putida
1.13.11.56 o-phenanthroline
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.56 0.15
-
 3-methylcatechol pH 6.2, 25°C Pseudomonas putida
1.13.11.56 0.27
-
 O2 pH 6.2, 25°C Pseudomonas putida
1.13.11.56 0.28
-
 1,2-dihydroxynaphthalene pH 6.2, 25°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.56 Fe2+ required. Other metal ions (Fe3+, C02+, Ca2+, Cu2+, Hg2+, Ni2+, Zn2+, Mg2+) could not replace Fe2+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.56 19000
-
 x * 19000, SDS-PAGE Pseudomonas putida
1.13.11.56 275000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.56 Pseudomonas putida
-
 NCIB 9816
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.56
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.56 culture condition:naphthalene-grown cell
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.56 1,2-dihydroxynaphthalene + O2 2-hydroxychromene-2-carboxylic acid may result from cycization of a ring fission product before release from the enzyme Pseudomonas putida 2-hydroxy-2H-chromene-2-carboxylate
-
 ?
1.13.11.56 3-methylcatechol + O2 5% of the activity with 1,2-dihydroxynaphthalene. The product 2-hydroxy-6-oxoheptadienoic acid is not structurally analogous to 2-hydroxychromene-2-carboxylic acid Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
 ?
1.13.11.56 4-methylcatechol + O2 2% of the activity with 1,2-dihydroxynaphthalene Pseudomonas putida ?
-
 ?
1.13.11.56 additional information purified enzyme shows no activity with catechol Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.56 ? x * 19000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.56 1,2-dihydroxynaphthalene oxygenase
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.56 6.5
-
-
 Pseudomonas putida