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Literature summary extracted from

  • Watanabe, H.; Nishimoto, T.; Chaen, H.; Fukuda, S.
    A novel glucanotransferase that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage (2007), J. Appl. Glycosci., 54, 109-118.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.248 Ca2+ enhances thermal stability Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.248 Cu2+ 1 mM, 80% residual acitvity Niallia circulans
2.4.1.248 EDTA 1 mM, 86% residual acitvity Niallia circulans
2.4.1.248 Fe2+ 1 mM, 94% residual acitvity Niallia circulans
2.4.1.248 Fe3+ 1 mM, 82% residual acitvity Niallia circulans
2.4.1.248 Hg2+ 1 mM, 55% residual acitvity Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.248 Pb2+ 1 mM, 130% of initial activity Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.248 106000
-
 2 * 106000, SDS-PAGE Niallia circulans
2.4.1.248 184000
-
 gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.248 Niallia circulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.248
-
 Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.248 6.1
-
 pH 6.0, 45 Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.248 amylose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 27.2% ?
2.4.1.248 glycogen
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 14.6% ?
2.4.1.248 maltoheptaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 15.8% ?
2.4.1.248 maltohexaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 12.7% ?
2.4.1.248 maltopentaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 8.9% ?
2.4.1.248 maltotetraose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 4.3% ?
2.4.1.248 maltotriose poor substrate Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 0.9% ?
2.4.1.248 additional information enzyme acts on maltooligosaccharides that have degrees of polymerization of 3 or more, amylose, and soluble starch, to produce cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) by an intramolecular alpha-1,6-glycosyl transfer reaction. Enzyme additionally catalyzes the transfer of part of a linear oligosaccharide to another oligosaccharide by an intermolecular alpha-1,4-glycosyl transfer reaction Niallia circulans ?
-
 ?
2.4.1.248 soluble starch
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 26.5% ?

Subunits

EC Number Subunits Comment Organism
2.4.1.248 dimer 2 * 106000, SDS-PAGE Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.248 isocyclomaltooligosaccharide glucanotransferase
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.248 50
-
-
 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.248 35
-
 stable up to Niallia circulans
2.4.1.248 40
-
 in presence of 1 mM Ca2+, stable up to Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.248 4.5 8
-
 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.248 4.5 9
-
 Niallia circulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.248 Niallia circulans isoelectric focusing
-
 7.5