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Literature summary extracted from

  • Bayer, T.S.; Widmaier, D.M.; Temme, K.; Mirsky, E.A.; Santi, D.V.; Voigt, C.A.
    Synthesis of methyl halides from biomass using engineered microbes (2009), J. Am. Chem. Soc., 131, 6508-6515.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Oryza sativa
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Brassica rapa
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Vitis vinifera
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Burkholderia pseudomallei
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Paraburkholderia xenovorans
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Burkholderia thailandensis
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Paraburkholderia phytofirmans
2.1.1.165 synthesis producing methyl halides from non-food agricultural resources by using a symbiotic co-culture of an engineered yeast and the cellulolytic bacterium Actinotalea fermentans, methyl halide production from unprocessed switchgrass (Panicum virgatum), corn stover, sugar cane bagasse, and poplar (Populus sp.). Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels Batis maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.165 synthesis of all putative methyl halide transferase from the NCBI sequence database and assay of methyl halide production in Escherichia coli Oryza sativa
2.1.1.165 synthesis of all putative methyl halide transferase from the NCBI sequence database and assay of methyl halide production in Escherichia coli Paraburkholderia xenovorans
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Brassica rapa
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Vitis vinifera
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Burkholderia pseudomallei
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Burkholderia thailandensis
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Paraburkholderia phytofirmans
2.1.1.165 synthesis of all putative methyl halide transferases from the NCBI sequence database and assay of methyl halide production in Escherichia coli Batis maritima

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Batis maritima
-
-
-
2.1.1.165 Brassica rapa
-
chinensis
-
2.1.1.165 Burkholderia pseudomallei
-
-
-
2.1.1.165 Burkholderia thailandensis
-
-
-
2.1.1.165 Oryza sativa
-
-
-
2.1.1.165 Paraburkholderia phytofirmans
-
-
-
2.1.1.165 Paraburkholderia xenovorans
-
-
-
2.1.1.165 Vitis vinifera
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Oryza sativa S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Vitis vinifera S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Paraburkholderia xenovorans S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Paraburkholderia phytofirmans S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Batis maritima S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide very low activity Brassica rapa S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide very low activity Burkholderia pseudomallei S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide very low activity Burkholderia thailandensis S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride
-
Brassica rapa S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride
-
Paraburkholderia phytofirmans S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride
-
Batis maritima S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride very low activity Vitis vinifera S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride very low activity Paraburkholderia xenovorans S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Oryza sativa S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Brassica rapa S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Vitis vinifera S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Burkholderia pseudomallei S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Paraburkholderia xenovorans S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Burkholderia thailandensis S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Paraburkholderia phytofirmans S-adenosyl-L-homocysteine + methyl iodide
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Batis maritima S-adenosyl-L-homocysteine + methyl iodide
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.165 methyl halide transferase
-
Oryza sativa
2.1.1.165 methyl halide transferase
-
Brassica rapa
2.1.1.165 methyl halide transferase
-
Vitis vinifera
2.1.1.165 methyl halide transferase
-
Burkholderia pseudomallei
2.1.1.165 methyl halide transferase
-
Paraburkholderia xenovorans
2.1.1.165 methyl halide transferase
-
Burkholderia thailandensis
2.1.1.165 methyl halide transferase
-
Paraburkholderia phytofirmans
2.1.1.165 methyl halide transferase
-
Batis maritima
2.1.1.165 MHT
-
Oryza sativa
2.1.1.165 MHT
-
Brassica rapa
2.1.1.165 MHT
-
Vitis vinifera
2.1.1.165 MHT
-
Burkholderia pseudomallei
2.1.1.165 MHT
-
Paraburkholderia xenovorans
2.1.1.165 MHT
-
Burkholderia thailandensis
2.1.1.165 MHT
-
Paraburkholderia phytofirmans
2.1.1.165 MHT
-
Batis maritima