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Literature summary extracted from

  • Chen, C.J.; Huang, C.Y.; Huang, J.K.; Lin, C.Y.; Lin, C.T.
    Cloning, expression, and purification of a functional glutathione reductase from sweet potato (Ipomoea batatas [L.] Lam): Kinetic studies and characterization (2009), J. Agric. Food Chem., 57, 4403-4408.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 expressed in Saccharomyces cerevisiae Ipomoea batatas

General Stability

EC Number General Stability Organism
1.8.1.7 active in the presence of imidazole up to 0.8 M, the native enzyme is resistant to digestion by trypsin or chymotrypsin Ipomoea batatas

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 Cu2+ in the presence of 1 mM Cu2+, approximately 5% of residual activity is detected Ipomoea batatas
1.8.1.7 additional information not inhibited by Ca2+, addition of imidazole up to 0.8 M has no significant effect on the activity Ipomoea batatas
1.8.1.7 Zn2+ in the presence of 1 mM Zn2+, approximately 55% of residual activity is detected Ipomoea batatas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.056
-
 NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
1.8.1.7 0.114
-
 glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 54600
-
 quadrupol time of flight electrospray ionization mass spectrometry Ipomoea batatas
1.8.1.7 54800
-
 1 * 54800, active monomeric form, SDS-PAGE Ipomoea batatas

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Ipomoea batatas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 Ni-NTA affinity column chromatography Ipomoea batatas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 root
-
 Ipomoea batatas
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Ipomoea batatas glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 monomer 1 * 54800, active monomeric form, SDS-PAGE Ipomoea batatas

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 EC 1.6.4.2 formerly Ipomoea batatas
1.8.1.7 glutathione reductase
-
 Ipomoea batatas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 70
-
 the protein's half-life of deactivation at 70°C is 3.3 min Ipomoea batatas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.7 50.14
-
 glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
1.8.1.7 53.72
-
 NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.7 6 11
-
 Ipomoea batatas

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.1.7 4
-
 glutathione reductase has little or no activity at pH 4.0 or lower Ipomoea batatas

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD
-
 Ipomoea batatas
1.8.1.7 NADPH
-
 Ipomoea batatas

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.7 439.8
-
 glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
1.8.1.7 959.3
-
 NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas