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Literature summary extracted from

  • Yang, S.; Qiaojuan, Y.; Jiang, Z.; Fan, G.; Wang, L.
    Biochemical characterization of a novel thermostable beta-1,3-1,4-glucanase (lichenase) from Paecilomyces thermophila (2008), J. Agric. Food Chem., 56, 5345-5351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.73 Co2+
-
Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 additional information the J18 strain produces the maximum level of extracellular beta-glucanase (135.6 U/ml) when grown in a medium containing corncob (5%, w/v) at 50°C for 4 days Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 N-bromosuccinimide
-
Paecilomyces sp. (in: Eurotiomycetes)

Application

EC Number Application Comment Organism
3.2.1.73 industry properties make the enzyme highly suitable for industrial applications Paecilomyces sp. (in: Eurotiomycetes)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Ag+ inhibits the enzyme activity at 4 mM by 56.9% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 beta-mercaptoethanol inhibits the enzyme activity at 4 mM by 82.7% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Ca2+ inhibits the enzyme activity at 4 mM by 55.5% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Cu2+ completely inhibits the enzyme activity at 4 mM Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 DTT inhibits the enzyme activity at 4 mM by 82.3% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 EDTA inhibits the enzyme activity at 4 mM by 43.4% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Fe2+ inhibits the enzyme activity at 4 mM by 14.9% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Fe3+ inhibits the enzyme activity at 4 mM by 13.5% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Hg2+ completely inhibits the enzyme activity at 4 mM Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Mg2+ inhibits the enzyme activity at 4 mM by 52.9% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Mn2+ completely inhibits the enzyme activity at 4 mM Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Na+ inhibits the enzyme activity at 4 mM by 47.3%; inhibits the enzyme activity at 4 mM by 80.3% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Ni2+ inhibits the enzyme activity at 4 mM by 11.5% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 SDS inhibits the enzyme activity at 4 mM by 88.2% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Sr2+ inhibits the enzyme activity at 4 mM by 49% Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Zn2+ inhibits the enzyme activity at 4 mM by 11.6% Paecilomyces sp. (in: Eurotiomycetes)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 31690
-
mass spectrometry Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 34600
-
gel filtration Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 38600
-
1 * 38600, SDS-PAGE Paecilomyces sp. (in: Eurotiomycetes)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Paecilomyces sp. (in: Eurotiomycetes)
-
-
-
3.2.1.73 Paecilomyces sp. (in: Eurotiomycetes) J18
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 by ammonium sulfate precipitation and two steps of ion-exchange chromatographies. Purified 122.5fold with an apparent homogeneity and a recovery yield of 8.9% Paecilomyces sp. (in: Eurotiomycetes)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 50.9
-
crude culture supernatant Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 6242
-
122.5fold purified enzyme Paecilomyces sp. (in: Eurotiomycetes)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
Paecilomyces sp. (in: Eurotiomycetes) beta-glucan oligosaccharides
-
?
3.2.1.73 barley beta-glucan + H2O
-
Paecilomyces sp. (in: Eurotiomycetes) J18 beta-glucan oligosaccharides
-
?
3.2.1.73 lichenan + H2O
-
Paecilomyces sp. (in: Eurotiomycetes) lichenan oligosaccharides
-
?
3.2.1.73 lichenan + H2O
-
Paecilomyces sp. (in: Eurotiomycetes) J18 lichenan oligosaccharides
-
?
3.2.1.73 additional information beta-glucanase does not act toward avicel, carboxymethylcellulose, cellulose, filter paper, beta-1,3-glucan, hydroxyethyl-cellulose, laminarin, starch, pullulan, birchwood xylan, locust bean gum, cellobiose, and xylobiose. No activity for p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-galactopyranoside, and p-nitrophenyl-beta-D-mannopyranoside Paecilomyces sp. (in: Eurotiomycetes) ?
-
?
3.2.1.73 additional information beta-glucanase does not act toward avicel, carboxymethylcellulose, cellulose, filter paper, beta-1,3-glucan, hydroxyethyl-cellulose, laminarin, starch, pullulan, birchwood xylan, locust bean gum, cellobiose, and xylobiose. No activity for p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-galactopyranoside, and p-nitrophenyl-beta-D-mannopyranoside Paecilomyces sp. (in: Eurotiomycetes) J18 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 monomer 1 * 38600, SDS-PAGE Paecilomyces sp. (in: Eurotiomycetes)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3-1,4-glucanase
-
Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 beta-glucanase
-
Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 endo-beta-1,3-1,4-glucanase
-
Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 Lichenase
-
Paecilomyces sp. (in: Eurotiomycetes)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 70
-
-
Paecilomyces sp. (in: Eurotiomycetes)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 60 80
-
Paecilomyces sp. (in: Eurotiomycetes)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 65
-
for 30 min Paecilomyces sp. (in: Eurotiomycetes)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 81.6
-
Barley beta-glucan calculated as D-glucose units released from beta-glucan Paecilomyces sp. (in: Eurotiomycetes)
3.2.1.73 166.4
-
lichenan calculated as D-glucose units released from lichenan Paecilomyces sp. (in: Eurotiomycetes)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 7
-
-
Paecilomyces sp. (in: Eurotiomycetes)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 4 10
-
Paecilomyces sp. (in: Eurotiomycetes)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.73 Paecilomyces sp. (in: Eurotiomycetes) isoelectric focusing
-
4.2