EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | 1-deoxymannojirimycin | binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri | |
3.2.1.24 | swainsonine | binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.0073 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.00731 | - |
4-methylumbelliferyl alpha-D-mannopyranoside | pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3 | Aspergillus fischeri | |
3.2.1.24 | 0.0826 | - |
4-nitrophenyl-alpha-D-mannopyranoside | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.08265 | - |
4-nitrophenyl-alpha-D-mannopyranoside | - |
Aspergillus fischeri | |
3.2.1.24 | 0.839 | - |
Manalpha(1-3)Man | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.839 | - |
Man-alpha(1-3)Man | pH 6.5, 50°C, 20 min | Aspergillus fischeri | |
3.2.1.24 | 1.116 | - |
Manalpha(1-2)Man | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 1.455 | - |
Man-alpha(1-6)Man | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 1.455 | - |
Man-alpha(1-6)Man | pH 6.5, 50°C, 20 min | Aspergillus fischeri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.24 | Aspergillus fischeri | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.24 | - |
Aspergillus fischeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.24 | 4-methylumbelliferyl alpha-D-mannopyranoside + H2O | pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3 | Aspergillus fischeri | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
3.2.1.24 | 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Aspergillus fischeri | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
3.2.1.24 | 4-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Aspergillus fischeri | 4-nitrophenol + D-mannose | - |
? | |
3.2.1.24 | 4-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Aspergillus fischeri | 4-nitrophenyl + alpha-D-mannose | - |
? | |
3.2.1.24 | Man-alpha(1-3)Man + H2O | pH 6.5, 50°C, 20 min | Aspergillus fischeri | alpha-D-mannose | - |
? | |
3.2.1.24 | Man-alpha(1-6)Man + H2O | pH 6.5, 50°C, 20 min | Aspergillus fischeri | alpha-D-mannose | - |
? | |
3.2.1.24 | Manalpha(1-2)Man + H2O | - |
Aspergillus fischeri | D-mannose | - |
? | |
3.2.1.24 | Manalpha(1-3)Man + H2O | - |
Aspergillus fischeri | D-mannose | - |
? | |
3.2.1.24 | Manalpha(1-6)Man + H2O | - |
Aspergillus fischeri | D-mannose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.24 | class II alpha-mannosidase | - |
Aspergillus fischeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 50 | - |
- |
Aspergillus fischeri |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 30 | 50 | 15 min | Aspergillus fischeri |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 30 | 50 | fully stable, no inactivation, no denaturation | Aspergillus fischeri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 6.5 | - |
- |
Aspergillus fischeri |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.101 | - |
swainsonine | low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.101 | - |
swainsonine | pH 6.5, 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.286 | - |
1-deoxymannojirimycin | low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri | |
3.2.1.24 | 0.286 | - |
1-deoxymannojirimycin | pH 6.5, 50°C | Aspergillus fischeri |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.11 | - |
low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri | swainsonine | |
3.2.1.24 | 0.284 | - |
low binding up to 40°C, increased binding at 50°C | Aspergillus fischeri | 1-deoxymannojirimycin |