EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | gene DUT, recombinant expression | Trypanosoma brucei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.23 | additional information | down-regulation of the enzyme activity by RNAi greatly reduces cell proliferation and increases the intracellular levels of dUTP. Defects in growth can be partially reverted by the addition of exogenous thymidine. dUTPase-depleted cells present hypersensitivity to methotrexate, a drug that increases the intracellular pools of dUTP, and enhanced uracil-DNA glycosylase activity, the first step in base excision repair, overview | Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.00179 | - |
dUTP | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.23 | additional information | analysis of subcellular localization of dUTPase, no localization in the mitochondrion or kinetoplast | Trypanosoma brucei | - |
- |
3.6.1.23 | nucleus | - |
Trypanosoma brucei | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required | Trypanosoma brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Trypanosoma brucei | dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | Trypanosoma brucei 427 | dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA | dUMP + diphosphate | - |
? | |
3.6.1.23 | additional information | Trypanosoma brucei | procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation | ? | - |
? | |
3.6.1.23 | additional information | Trypanosoma brucei 427 | procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Trypanosoma brucei | - |
gene DUT with T49 and E248 | - |
3.6.1.23 | Trypanosoma brucei 427 | - |
gene DUT with T49 and E248 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.23 | additional information | the enzyme is expressed in different life stages. Trypanosoma brucei brucei bloodstream form cell line S16 and the procyclic cell line 449 | Trypanosoma brucei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Trypanosoma brucei | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA | Trypanosoma brucei | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Trypanosoma brucei 427 | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA | Trypanosoma brucei 427 | dUMP + diphosphate | - |
? | |
3.6.1.23 | additional information | procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation | Trypanosoma brucei | ? | - |
? | |
3.6.1.23 | additional information | procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation | Trypanosoma brucei 427 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | dimer | - |
Trypanosoma brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Trypanosoma brucei |
3.6.1.23 | dUTPase | - |
Trypanosoma brucei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 37 | - |
assay at | Trypanosoma brucei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 9.7 | - |
dUTP | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 7.4 | - |
assay at | Trypanosoma brucei |