Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, H.; Lu, L.; Xiao, M.; Wang, Q.; Lu, Y.; Liu, C.; Wang, P.; Kumagai, H.; Yamamoto, K.
    Cloning and characterization of a novel alpha-galactosidase from Bifidobacterium breve 203 capable of synthesizing Gal-alpha-1,4 linkage (2008), FEMS Microbiol. Lett., 285, 278-283.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 EDTA 16% increase in activity Bifidobacterium breve
3.2.1.22 NH4+ 23% increase in activity Bifidobacterium breve

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22
-
 Bifidobacterium breve

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ strong Bifidobacterium breve
3.2.1.22 Cu2+ strong Bifidobacterium breve
3.2.1.22 Hg2+ strong Bifidobacterium breve

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.27
-
 4-nitrophenyl-alpha-D-galactopyranoside
-
 Bifidobacterium breve
3.2.1.22 4.3
-
 melibiose
-
 Bifidobacterium breve

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80500
-
 2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve
3.2.1.22 81500
-
 2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve
3.2.1.22 152000
-
 PAGE Bifidobacterium breve

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Bifidobacterium breve Q2XQ11
-
-
3.2.1.22 Bifidobacterium breve 203 Q2XQ11
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 2-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 2-nitrophenol + D-galactopyranose 16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3.2.1.22 2-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 203 2-nitrophenol + D-galactopyranose 16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3.2.1.22 3-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 3-nitrophenol + D-galactopyranose 5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3.2.1.22 3-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 203 3-nitrophenol + D-galactopyranose 5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.22 melibiose + H2O
-
 Bifidobacterium breve D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Bifidobacterium breve 203 D-galactose + D-glucose
-
 ?
3.2.1.22 additional information enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors Bifidobacterium breve ?
-
 ?
3.2.1.22 additional information no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside Bifidobacterium breve ?
-
 ?
3.2.1.22 additional information enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors Bifidobacterium breve 203 ?
-
 ?
3.2.1.22 additional information no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside Bifidobacterium breve 203 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
 Bifidobacterium breve

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50
-
 stable below Bifidobacterium breve

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5.5
-
-
 Bifidobacterium breve

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 4.5 9
-
 Bifidobacterium breve