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Literature summary extracted from

  • Thomas, G.; Potter, L.; Cole, J.A.
    The periplasmic nitrate reductase from Escherichia coli: a heterodimeric molybdoprotein with a double-arginine signal sequence and an unusual leader peptide cleavage site (1999), FEMS Microbiol. Lett., 174, 167-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.9.6.1
-
 Escherichia coli K-12

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.9.6.1 periplasm
-
 Escherichia coli K-12
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.9.6.1 Mo molybdoprotein Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.9.6.1 16000
-
 1 * 90000 (NapA) + 1 * 16000 (NapB), Nap activity is lost rapidly during the separation of NapA from NapB by anion exchange chromatography, SDS-PAGE Escherichia coli K-12
1.9.6.1 90000
-
 1 * 90000 (NapA) + 1 * 16000 (NapB), Nap activity is lost rapidly during the separation of NapA from NapB by anion exchange chromatography, SDS-PAGE Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
1.9.6.1 Escherichia coli K-12 P33937 and P0ABL3 P33937: napA, P0ABL3: napB
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.9.6.1 proteolytic modification the pre-NapA leader sequence is both unexpectedly long and, unless two successive proteolysis steps are involved, is cleaved at the unprecedented sequence G-Q-Q Escherichia coli K-12

Storage Stability

EC Number Storage Stability Organism
1.9.6.1 4°C, about 90% of the activity is lost after 48 h, no activity remains after 4 days Escherichia coli K-12

Subunits

EC Number Subunits Comment Organism
1.9.6.1 heterodimer 1 * 90000 (NapA) + 1 * 16000 (NapB), Nap activity is lost rapidly during the separation of NapA from NapB by anion exchange chromatography, SDS-PAGE Escherichia coli K-12

Synonyms

EC Number Synonyms Comment Organism
1.9.6.1 NapA
-
 Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.9.6.1 heme subunit NapB is a diheme cytochrome c Escherichia coli K-12