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Literature summary extracted from

  • Hwang, E.Y.; Huh, J.W.; Choi, M.M.; Choi, S.Y.; Hong, H.N.; Cho, S.W.
    Inhibitory effects of gallic acid and quercetin on UDP-glucose dehydrogenase activity (2008), FEBS Lett., 582, 3793-3797.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.22 medicine possibility that gallic acid and quercetin may modulate human breast cancer cell proliferation by inhibiting UGDH Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.22 gallic acid is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control Homo sapiens
1.1.1.22 quercetin shows a competitive inhibition and a mixed-type inhibition with respect to UDP-glucose and NAD+, decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Quercetin exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of quercetin, the activity remaining after 30 min is 20% that of control Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.22 0.017
-
UDP-glucose
-
Homo sapiens
1.1.1.22 0.13
-
NAD+
-
Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.22 MCF-7 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.22 0.00051
-
lysates of cells treated with 0.3 mM gallic acid Homo sapiens
1.1.1.22 0.00061
-
lysates of cells treated with 0.3 mM quercetin Homo sapiens
1.1.1.22 0.0015
-
control lysates Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 UDP-glucose + 2 NAD+ + H2O
-
Homo sapiens UDP-glucuronate + 2 NADH + 2 H+
-
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Synonyms

EC Number Synonyms Comment Organism
1.1.1.22 UDP-glucose dehydrogenase
-
Homo sapiens
1.1.1.22 UGDH
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.22 0.07
-
quercetin with respect to NAD+ Homo sapiens
1.1.1.22 0.083
-
quercetin with respect to UDP-glucose Homo sapiens
1.1.1.22 0.744
-
gallic acid with respect to UDP-glucose Homo sapiens
1.1.1.22 1.804
-
gallic acid with respect to NAD+ Homo sapiens