EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.22 | medicine | possibility that gallic acid and quercetin may modulate human breast cancer cell proliferation by inhibiting UGDH | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.22 | gallic acid | is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control | Homo sapiens | |
1.1.1.22 | quercetin | shows a competitive inhibition and a mixed-type inhibition with respect to UDP-glucose and NAD+, decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Quercetin exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of quercetin, the activity remaining after 30 min is 20% that of control | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.22 | 0.017 | - |
UDP-glucose | - |
Homo sapiens | |
1.1.1.22 | 0.13 | - |
NAD+ | - |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.22 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.22 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.22 | MCF-7 cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.22 | 0.00051 | - |
lysates of cells treated with 0.3 mM gallic acid | Homo sapiens |
1.1.1.22 | 0.00061 | - |
lysates of cells treated with 0.3 mM quercetin | Homo sapiens |
1.1.1.22 | 0.0015 | - |
control lysates | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.22 | UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.22 | UDP-glucose dehydrogenase | - |
Homo sapiens |
1.1.1.22 | UGDH | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.22 | 0.07 | - |
quercetin | with respect to NAD+ | Homo sapiens | |
1.1.1.22 | 0.083 | - |
quercetin | with respect to UDP-glucose | Homo sapiens | |
1.1.1.22 | 0.744 | - |
gallic acid | with respect to UDP-glucose | Homo sapiens | |
1.1.1.22 | 1.804 | - |
gallic acid | with respect to NAD+ | Homo sapiens |