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Literature summary extracted from
- New roles of flavoproteins in molecular cell biology: histone demethylase LSD1 and chromatin (2009), FEBS J., 276, 4304-4312.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.66 | CoREST | the co-repressor CoREST stabilizes LSD1 and increases in vitro LSD1 activity by approximately twofold and is essential for LSD1-mediated demethylation in intact nucleosomes in vivo and for the in vitro demethylation of nucleosomal particles, binding structure, overview | Homo sapiens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.99.66 | enzyme complex LSD1-CoREST crystal structure in complex with a histone H3 peptide, overview | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.99.66 | K661A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.66 | nucleosome | - |
Homo sapiens | 786 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.66 | additional information | Homo sapiens | LSD1 interacts with CoREST, a co-repressor protein that binds REST and recruits other histone-modifying enzymes such as histone deacetylases 1 ? 2. The function of the LSD1CoRESThistone deacetylase subcomplex in transcriptional repression events is not limited to REST-regulated neuronal genes, but can be extended to other contexts such as hematopoietic differentiation and the telomerase reverse transcriptase genes | ? | - |
? |  |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | Drosophila melanogaster | - |
[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | Homo sapiens | - |
[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | Arabidopsis thaliana | - |
[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | Caenorhabditis elegans | - |
[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Drosophila melanogaster | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Caenorhabditis elegans | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.66 | Arabidopsis thaliana | - |
- |
- |
| 1.14.99.66 | Caenorhabditis elegans | - |
- |
- |
| 1.14.99.66 | Drosophila melanogaster | - |
- |
- |
| 1.14.99.66 | Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.66 | additional information | LSD1 interacts with CoREST, a co-repressor protein that binds REST and recruits other histone-modifying enzymes such as histone deacetylases 1 ? 2. The function of the LSD1CoRESThistone deacetylase subcomplex in transcriptional repression events is not limited to REST-regulated neuronal genes, but can be extended to other contexts such as hematopoietic differentiation and the telomerase reverse transcriptase genes | Homo sapiens | ? | - |
? | |
| 1.14.99.66 | additional information | LSD1 is specific for Lys4 of both mono- and dimethylated histone H3 using a highly specific recognition mechanism, LSD1-catalyzed reaction produces an imine intermediate that is hydrolyzed to the demethylated product and formaldehyde, Reduced flavin is reoxidized by molecular oxygen with the concomitant production of hydrogen peroxide, overview | Drosophila melanogaster | ? | - |
? | |
| 1.14.99.66 | additional information | LSD1 is specific for Lys4 of both mono- and dimethylated histone H3 using a highly specific recognition mechanism, LSD1-catalyzed reaction produces an imine intermediate that is hydrolyzed to the demethylated product and formaldehyde, Reduced flavin is reoxidized by molecular oxygen with the concomitant production of hydrogen peroxide, overview | Homo sapiens | ? | - |
? | |
| 1.14.99.66 | additional information | LSD1 is specific for Lys4 of both mono- and dimethylated histone H3 using a highly specific recognition mechanism, LSD1-catalyzed reaction produces an imine intermediate that is hydrolyzed to the demethylated product and formaldehyde, Reduced flavin is reoxidized by molecular oxygen with the concomitant production of hydrogen peroxide, overview | Arabidopsis thaliana | ? | - |
? | |
| 1.14.99.66 | additional information | LSD1 is specific for Lys4 of both mono- and dimethylated histone H3 using a highly specific recognition mechanism, LSD1-catalyzed reaction produces an imine intermediate that is hydrolyzed to the demethylated product and formaldehyde, Reduced flavin is reoxidized by molecular oxygen with the concomitant production of hydrogen peroxide, overview | Caenorhabditis elegans | ? | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | - |
Drosophila melanogaster | [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | - |
Homo sapiens | [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | - |
Arabidopsis thaliana | [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | - |
Caenorhabditis elegans | [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Drosophila melanogaster | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.99.66 | [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.99.66 | More | LSD1 is a multidomain protein | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.66 | histone demethylase | - |
Drosophila melanogaster |
| 1.14.99.66 | histone demethylase | - |
Homo sapiens |
| 1.14.99.66 | histone demethylase | - |
Arabidopsis thaliana |
| 1.14.99.66 | histone demethylase | - |
Caenorhabditis elegans |
| 1.14.99.66 | LSD1 | - |
Drosophila melanogaster |
| 1.14.99.66 | LSD1 | - |
Homo sapiens |
| 1.14.99.66 | LSD1 | - |
Arabidopsis thaliana |
| 1.14.99.66 | LSD1 | - |
Caenorhabditis elegans |
| 1.14.99.66 | More | LSD1 is a flavin-dependent amine axidase | Drosophila melanogaster |
| 1.14.99.66 | More | LSD1 is a flavin-dependent amine axidase | Homo sapiens |
| 1.14.99.66 | More | LSD1 is a flavin-dependent amine axidase | Arabidopsis thaliana |
| 1.14.99.66 | More | LSD1 is a flavin-dependent amine axidase | Caenorhabditis elegans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.66 | FAD | dependent on | Drosophila melanogaster | |
| 1.14.99.66 | FAD | dependent on | Homo sapiens | |
| 1.14.99.66 | FAD | dependent on | Arabidopsis thaliana | |
| 1.14.99.66 | FAD | dependent on | Caenorhabditis elegans | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.99.66 | physiological function | LSD1 plays a role in gene expression regulation, it is a multidomain protein and its involvement in many diverse gene-expression programs is strictly related to its domain organization | Homo sapiens |
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