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Literature summary extracted from

  • Balconi, E.; Pennati, A.; Crobu, D.; Pandini, V.; Cerutti, R.; Zanetti, G.; Aliverti, A.
    The ferredoxin-NADP+ reductase/ferredoxin electron transfer system of Plasmodium falciparum (2009), FEBS J., 276, 3825-3836.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 overexpressed in Escherichia coli Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0008
-
 oxidized ferredoxin Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor Plasmodium falciparum
1.18.1.2 0.0055
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
1.18.1.2 0.01
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
1.18.1.2 0.05
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
 Plasmodium falciparum NADP+ + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
 ?
1.18.1.2 NADPH + K3Fe(CN)6
-
 Plasmodium falciparum NADP+ + reduced K3Fe(CN)6
-
 r
1.18.1.2 NADPH + oxidized cytochrome c
-
 Plasmodium falciparum NADP+ + reduced cytochrome c
-
 r
1.18.1.2 NADPH + oxidized ferredoxin
-
 Plasmodium falciparum NADP+ + reduced ferredoxin
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH + H+
-
 Plasmodium falciparum reduced ferredoxin + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 More Under oxidizing conditions, Plasmodium falciparum FNR constitutes an inactive dimeric form stabilized by an intermolecular disulfide bond. Monomer-dimer interconversion can be controlled by oxidizing and reducing agents that are possibly present within the apicoplast, such as H2O2, glutathione, and lipoate. Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
 Plasmodium falciparum
1.18.1.2 FNR
-
 Plasmodium falciparum
1.18.1.2 PfFNR ferredoxin-NADP+ reductase of Plasmodium falciparum Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 7.5
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
1.18.1.2 65
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum
1.18.1.2 85
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 1200
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
1.18.1.2 1300
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum
1.18.1.2 8000
-
 Ferredoxin Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor Plasmodium falciparum
1.18.1.2 8500
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum