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Literature summary extracted from
- Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation (2009), FEBS J., 276, 1891-1899.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.22.1 | additional information | proteolytic processing of recombinant DNase II occurs in the lysosome, which is not involved in the activation of DNase II. The processed enzyme is relatively stable after it is formed | Sus scrofa |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.22.1 | cDNA of pDNase II cloned in the pcDNA3 vector and transfected into HEK 293T cells | Sus scrofa |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.22.1 | lysosome | post-translational processing of the DNase II to produce the alpha1, beta and alpha2 subunits occurs in the lysosome | Sus scrofa | 5764 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.22.1 | 35000 | - |
Western blot analysis, alpha subunit of DNase II | Sus scrofa |
| 3.1.22.1 | 38210 | - |
sequence analysis | Sus scrofa |
| 3.1.22.1 | 46500 | - |
Western blot analysis or calculated (addition of the mass of sugar contents to the sequence-derived mass) | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.22.1 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.22.1 | to homogeneity by gel filtration, 74.3fold purified | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.22.1 | spleen | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.22.1 | 4.4 | - |
1fold purified | Sus scrofa |
| 3.1.22.1 | 327.1 | - |
74.3fold purified | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.22.1 | DNA + H2O | - |
Sus scrofa | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.22.1 | DNase II | - |
Sus scrofa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.22.1 | 4.7 | - |
hydrolyzes DNA under acidic conditions | Sus scrofa |
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Release 2023.1 (January 2023)
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