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Literature summary extracted from

  • Yang, Q.; Liu, T.; Liu, F.; Qu, M.; Qian, X.
    A novel beta-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenee) (2008), FEBS J., 275, 5690-5702.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 additional information structural elements of substrates, the 2-acetamido group and the beta-glycoside bond linkage, are essential for its activity. Transcriptional level increases dramatically before the molting stage Ostrinia furnacalis

Application

EC Number Application Comment Organism
3.2.1.52 additional information Hex1 may be mostly involved in insect chitin catabolism and may be a specific target for pesticide development Ostrinia furnacalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.29
-
 4-nitrophenyl-beta-N-acetyl-D-glucosaminide
-
 Ostrinia furnacalis
3.2.1.52 0.3
-
 GlcNAcbeta(1-4)GlcNAc
-
 Ostrinia furnacalis
3.2.1.52 0.43
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
 Ostrinia furnacalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 65500
-
 2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis
3.2.1.52 67000
-
 2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis
3.2.1.52 128000
-
 gel filtration Ostrinia furnacalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Ostrinia furnacalis Q06GJ0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 1468fold to homogeneity with an activity yield of 20% by four column chromatography steps (anion exchange chromatography and gel filtration) Ostrinia furnacalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 additional information fifth instar larva integument Ostrinia furnacalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 0.017
-
 crude extract Ostrinia furnacalis
3.2.1.52 25
-
 1468fold purified enzyme Ostrinia furnacalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Ostrinia furnacalis 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Ostrinia furnacalis 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAc + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.52 additional information it can not hydrolyze the complex N-glycan substrate (GlcNAcbeta-1,2Manalpha-1,6)(GlcNAcbeta-1,2Manalpha-1,3)Manbeta-1,4GlcNAcbeta-1,4GlcNAc-PA as well as the long polymer chitin Ostrinia furnacalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 homodimer 2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 beta-N-acetyl-D-hexosaminidase
-
 Ostrinia furnacalis
3.2.1.52 Hex1
-
 Ostrinia furnacalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 30
-
-
 Ostrinia furnacalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 40
-
 stable for 30 min Ostrinia furnacalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 708
-
 4-nitrophenyl-beta-N-acetyl-D-glucosaminide
-
 Ostrinia furnacalis
3.2.1.52 766
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
 Ostrinia furnacalis
3.2.1.52 934
-
 GlcNAcbeta(1-4)GlcNAc
-
 Ostrinia furnacalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 7
-
-
 Ostrinia furnacalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 7
-
 stable for 30 min Ostrinia furnacalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.52 Ostrinia furnacalis IEF electrophoresis
-
 4.7