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Literature summary extracted from

  • Engeland K, Kindl H.
    Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA (1991), Eur. J. Biochem., 200, 171-178.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.119 0.0095
-
 2-trans-decenoyl-CoA pH 8.0, 25°C Cucumis sativus
4.2.1.119 0.11
-
 2-trans-butenoyl-CoA pH 8.0, 25°C Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.119 peroxisome D-specific 2-trans-enoyl-CoA hydratase is exclusively located in Cucumis sativus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.119 33000
-
 2 * 33000, SDS-PAGE Cucumis sativus
4.2.1.119 65000
-
 gel filtration Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Cucumis sativus
-
 L. cv. Chinesische Schlangengurken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.119
-
 Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.119 cotyledon fat-degrading Cucumis sativus
-
4.2.1.119 seedling
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.119 259
-
-
 Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 2-trans-butenoyl-CoA + H2O
-
 Cucumis sativus (3R)-hydroxybutanoyl-CoA
-
 ?
4.2.1.119 2-trans-decenoyl-CoA + H2O
-
 Cucumis sativus (3R)-3-hydroxydecanoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.119 homodimer 2 * 33000, SDS-PAGE Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 D-(-)-3-hydroxyacyl-CoA hydro-lyase
-
 Cucumis sativus
4.2.1.119 D-3-hydroxyacyl-CoA hydro-lyase
-
 Cucumis sativus
4.2.1.119 D-specific 2-trans-enoyl-CoA hydratase
-
 Cucumis sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.119 additional information
-
 additional information kcat/Km for 2-trans-decenoyl-CoA is 24 microM/s, kcat/Km for 2-trans-butenoyl-CoA is 0.44 microM/s Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.119 7.5 8
-
 Cucumis sativus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.119 Cucumis sativus around, estimated by comparing the elution of cationic enzymes on carboxymethyl-cellulose
-
 9