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Literature summary extracted from

  • Sode, K.; Tsugawa, W.; Yamazaki, T.; Watanabe, M.; Ogasawara, N.; Tanaka, M.
    A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures (1996), Enzyme Microb. Technol., 19, 82-85.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.9 membrane
-
 Burkholderia cepacia 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 43000
-
 after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
1.1.5.9 67000
-
 after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia
1.1.5.9 67000
-
 after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
1.1.5.9 85000
-
 after preincubation at 70°C for 30 min, native PAGE Burkholderia cepacia
1.1.5.9 350000
-
 native PAGE Burkholderia cepacia
1.1.5.9 380000
-
 gel filtration chromatography Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Burkholderia cepacia
-
 bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9
-
 Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.9 6
-
 at 75°C after preincubation of the enzyme at 70°C for 30 min Burkholderia cepacia
1.1.5.9 15
-
 at 70°C Burkholderia cepacia
1.1.5.9 45
-
 at 45°C Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 heteromer after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
1.1.5.9 homooligomer after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 thermostable GDH
-
 Burkholderia cepacia
1.1.5.9 thermostable glucose dehydrogenase
-
 Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 45
-
 enzyme activity shows a second peak at 70°C with 30% of maximal activity Burkholderia cepacia
1.1.5.9 75
-
 after preincubating the enzyme at 70°C for 30 min Burkholderia cepacia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.9 20 85 20% of maximal activity at 20°C and 10% of maximal activity at 85°C, maximal activity is at 45°C with a second activity peak at 70°C (30% of maximal activity) Burkholderia cepacia
1.1.5.9 40 85 after preincubation of the enzyme at 70°C for 30 min: 15% of maximal activity at 40°C and 50% of maximal activity at 85°C, maximal activity at 75°C Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.9 5 70 enzyme is stable at temperatures between 5-37°C for 30 min, after incubating enzyme at 45°C for 30 min 40% of enzyme activity remains, at 70°C for 30 min 10% of enzyme activity remains Burkholderia cepacia
1.1.5.9 5 75 after preincubation at 70°C for 30 min the enzyme is stable at temperatures between 5-50°C for 30 min, after incubating enzyme at 70°C for 30 min 70% of activity remains Burkholderia cepacia