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Literature summary extracted from

  • Choudhury, R.; Noor, S.; Varadarajalu, L.; Punekar, N.
    Delineation of an in vivo inhibitor for Aspergillus glutamate dehydrogenase (2008), Enzyme Microb. Technol., 42, 151-159.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.1.4 molecular biology results implicate glutamate dehydrogenase and NADP-GDH in particular, as a key target of in vivo isophthalate inhibition during ammonium assimilation Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 isophthalate potent in vitro inhibitor Aspergillus niger
1.4.1.4 2,4-pyridinedicarboxylate inhibits less efficiently Aspergillus niger
1.4.1.4 2-Methyleneglutarate
-
 Aspergillus niger
1.4.1.4 dimethyl ester of isophthalic acid dimethyl ester of isophthalate (DMIP), but not isophthalate, inhibits Aspergillus niger growth on agar as well as in liquid culture. This is ascribed to the inability of isophthalate to enter fungal mycelia. Dimethyl ester of isophthalic acid is hydrolysed intracellularly to isophthalate. Subsequent to DMIP addition, intracellular isophthalate can be demonstrated. Addition of NH4+ to DMIP-treated Aspergillus niger mycelia results in intensive vacuolation, retraction of cytoplasm and autolysis Aspergillus niger
1.4.1.4 isophthalate potent in vitro inhibitor Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Aspergillus niger
-
-
-
1.4.1.4 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 NAD-GDH is best obtained from proline grown Aspergillus niger mycelia Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxoglutarate + NH4+ + NADPH
-
 Aspergillus niger L-glutamate + NADP+
-
 ?
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Aspergillus niger L-glutamate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 NAD-GDH
-
 Aspergillus niger
1.4.1.4 glutamate dehydrogenase
-
 Aspergillus niger
1.4.1.4 NADP-GDH
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.2 28
-
 assay at Aspergillus niger
1.4.1.4 28
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8
-
 assay at Aspergillus niger
1.4.1.4 8
-
 assay at Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NADH
-
 Aspergillus niger
1.4.1.4 NADPH
-
 Aspergillus niger