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Literature summary extracted from

  • Liu, M.F.; Wu, X.P.; Wang, X.L.; Yu, Y.L.; Wang, W.F.; Chen, Q.J.; Boireau, P.; Liu, M.Y.
    The functions of Deoxyribonuclease II in immunity and development (2008), DNA Cell Biol., 27, 223-228.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.22.1 additional information catalytic center of the human DNase II involves His113 and His295, as well as Lys115 and Lys295. The histidine at position 295 of the human sequence is critical for DNase II activity. The human DNase II is active as a contiguous polypeptide chain and has four potential glycosylated sites Homo sapiens
3.1.22.1 additional information His115, His132, and His297 play crucial roles in the function of porcine DNase II. His115 and His297 residues in porcine DNase II act as a general acid-base pair during catalysis, whereas residue His132 appears to be involved in proper protein folding. The porcine DNase II is processed into two non-covalently linked peptides of 35 and 10 kDa and has six potential glycosylated sites Sus scrofa
3.1.22.1 additional information the His in WLVHT is an active center Trichinella spiralis

Application

EC Number Application Comment Organism
3.1.22.1 medicine DNase II in macrophage lysosomes is responsible for definitive erythropoiesis in mouse fetal liver Mus musculus
3.1.22.1 medicine DNase II in Trichinella spiralis are secreted into circulation to cleave undigested DNA from apoptotic host cells, which reduces the innate immune response. The role played by DNase II in Trichinella spiralis may be a self-protective mechanism. DNase IIbeta or DNase II of Trichinella spiralis can be considered a candidate protein for therapy of autoimmune diseases, DNase IIĀ–deficient diseases, or cancer Trichinella spiralis
3.1.22.1 additional information DNase II enzyme and the ability to clear macromolecular DNA are essential for maintaining proper immune function in Drosophila Drosophila sp. (in: flies)
3.1.22.1 additional information the DNase II from the crown-of-thorns starfish Acanthaster planci is toxic Acanthaster planci

Protein Variants

EC Number Protein Variants Comment Organism
3.1.22.1 additional information DNase IIĀ–deficient mouse embryos accumulate undigested DNA in macrophages, which are present in all tissues, such as the thymus, kidney, interdigit, spleen, and liver. The accumulation of undegraded DNA also stimulates production of interferon-beta, and eventually causes death of the knockout mice. Peripheral blood of DNase II-/- embryos contain massive nucleated erythrocytes and no live DNase II-/- mice are born because of severe respiratory disturbance. Deficiency of the DNase II in adult mice is not lethal Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.22.1 RNAi expression of a DNase IIĀ–RNAi construct in flies results in a significant decrease in total hemocyte numbers Drosophila sp. (in: flies)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.22.1 lysosome DNase IIbeta resides in the lysosomes of cortical fiber cells, close to the organelle-free zone Mus musculus 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Acanthaster planci
-
-
-
3.1.22.1 Drosophila sp. (in: flies)
-
-
-
3.1.22.1 Haemonchus contortus
-
-
-
3.1.22.1 Homo sapiens
-
-
-
3.1.22.1 Mus musculus
-
-
-
3.1.22.1 Sus scrofa
-
-
-
3.1.22.1 Trichinella spiralis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.22.1 adult
-
 Mus musculus
-
3.1.22.1 embryo
-
 Mus musculus
-
3.1.22.1 interdigit
-
 Mus musculus
-
3.1.22.1 kidney
-
 Mus musculus
-
3.1.22.1 lens
-
 Mus musculus
-
3.1.22.1 liver
-
 Mus musculus
-
3.1.22.1 additional information cortical fiber cell Mus musculus
-
3.1.22.1 additional information muscle larvae stage, newborn stage and adult stage Trichinella spiralis
-
3.1.22.1 spleen
-
 Mus musculus
-
3.1.22.1 thymus
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.22.1 DNA + H2O
-
 Mus musculus 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.22.1 DNase II
-
 Drosophila sp. (in: flies)
3.1.22.1 DNase II
-
 Mus musculus
3.1.22.1 DNase II
-
 Homo sapiens
3.1.22.1 DNase II
-
 Sus scrofa
3.1.22.1 DNase II
-
 Haemonchus contortus
3.1.22.1 DNase II
-
 Trichinella spiralis
3.1.22.1 DNase II
-
 Acanthaster planci
3.1.22.1 DNase IIalpha
-
 Sus scrofa
3.1.22.1 DNase IIbeta
-
 Mus musculus
3.1.22.1 DNase IIbeta
-
 Trichinella spiralis