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Literature summary extracted from

  • Di Nardo, G.; Roggero, C.; Campolongo, S.; Valetti, F.; Trotta, F.; Gilardi, G.
    Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges (2009), Dalton Trans., 33, 6507-6512.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.1 biotechnology product is precursor of the industrially important compound adipic acid Acinetobacter radioresistens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1
-
 Acinetobacter radioresistens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.1 additional information immobilized enzyme on nanosponges Acinetobacter radioresistens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.002
-
 catechol free protein Acinetobacter radioresistens
1.13.11.1 0.0166
-
 catechol immobilized protein Acinetobacter radioresistens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron contains iron Acinetobacter radioresistens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter radioresistens
-
-
-
1.13.11.1 Acinetobacter radioresistens S13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Acinetobacter radioresistens

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 25°C, 11 days, complete inactivation for the immobilized protein Acinetobacter radioresistens
1.13.11.1 25°C, 4 days, residual activity 50% for the immobilized protein and 10% for the free form Acinetobacter radioresistens
1.13.11.1 25°C, 5 days, complete inactivation for the free enzyme Acinetobacter radioresistens
1.13.11.1 4°C, 10 days, 50% residual acitivity for the immobilized protein and only 10% residual activity of the free form protein Acinetobacter radioresistens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
 Acinetobacter radioresistens ?
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter radioresistens cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter radioresistens S13 cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 catechol 1,2-dioxygenase
-
 Acinetobacter radioresistens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 30
-
 free protein Acinetobacter radioresistens
1.13.11.1 50
-
 immobilized protein Acinetobacter radioresistens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.1 10 60 at 60°C still 70% residual activity of the immobilized enzyme form Acinetobacter radioresistens
1.13.11.1 30 40 complete loss of enzyme activity of the free form at 60°C Acinetobacter radioresistens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 40
-
 90 min, 60% residual activity for the immobilized protein versus 20% residual activity for the free enzyme Acinetobacter radioresistens
1.13.11.1 60
-
 15 min, 75% residual activity for the immobilized protein versus total inactivation of the free enzyme Acinetobacter radioresistens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8.5
-
 free protein Acinetobacter radioresistens
1.13.11.1 9.5
-
 immobilized protein Acinetobacter radioresistens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 6.5 10 below no enzyme activity, at pH 8.5 40% residual enzyme activity of the immobilized enzyme compared to inactive free form enzyme Acinetobacter radioresistens