EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus ferrooxidans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.9 | C142A | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
1.8.1.9 | C142S | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
1.8.1.9 | C145A | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
1.8.1.9 | C145S | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 33000 | - |
SDS-PAGE | Acidithiobacillus ferrooxidans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Acidithiobacillus ferrooxidans | - |
strain ATCC 23270 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | Ni-NTA affinity column chromatography | Acidithiobacillus ferrooxidans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 5.1 | - |
mutant enzyme C145S, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
1.8.1.9 | 5.5 | - |
mutant enzyme C142S, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
1.8.1.9 | 5.6 | - |
mutant enzyme C145A, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
1.8.1.9 | 6.4 | - |
mutant enzyme C142A, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
1.8.1.9 | 29.7 | - |
wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Acidithiobacillus ferrooxidans | thioredoxin + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | thioredoxin reductase | - |
Acidithiobacillus ferrooxidans |
1.8.1.9 | TrxR | - |
Acidithiobacillus ferrooxidans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | FAD | - |
Acidithiobacillus ferrooxidans | |
1.8.1.9 | NADPH | - |
Acidithiobacillus ferrooxidans |