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Literature summary extracted from

  • van den Wildenberg, S.M.; Tao, L.; Kapitein, L.C.; Schmidt, C.F.; Scholey, J.M.; Peterman, E.J.
    The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations (2008), Curr. Biol., 18, 1860-1864.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.3 expressed in Sf9 cells Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.3 520000
-
full-length enzyme Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.3 additional information Drosophila melanogaster during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation ?
-
?
5.6.1.3 additional information Drosophila melanogaster KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.3 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.3 Ni-NTA affinity column chromatography and Superose 6 gel filtration Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.3 embryo
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.3 ATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
?
5.6.1.3 additional information during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation Drosophila melanogaster ?
-
?
5.6.1.3 additional information KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone Drosophila melanogaster ?
-
?

Subunits

EC Number Subunits Comment Organism
5.6.1.3 homotetramer
-
Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
5.6.1.3 kinesin-5
-
Drosophila melanogaster
5.6.1.3 KLP61F
-
Drosophila melanogaster