EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.1.3 | expressed in Sf9 cells | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.6.1.3 | 520000 | - |
full-length enzyme | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.3 | additional information | Drosophila melanogaster | during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation | ? | - |
? | |
5.6.1.3 | additional information | Drosophila melanogaster | KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.3 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.1.3 | Ni-NTA affinity column chromatography and Superose 6 gel filtration | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.6.1.3 | embryo | - |
Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
5.6.1.3 | additional information | during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation | Drosophila melanogaster | ? | - |
? | |
5.6.1.3 | additional information | KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone | Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.6.1.3 | homotetramer | - |
Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.1.3 | kinesin-5 | - |
Drosophila melanogaster |
5.6.1.3 | KLP61F | - |
Drosophila melanogaster |