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Literature summary extracted from

  • Campos, E.; Facanha, A.R.; Costa, E.P.; da Silva Vaz, I.; Masuda, A.; Logullo, C.
    Exopolyphosphatases in nuclear and mitochondrial fractions during embryogenesis of the hard tick Rhipicephalus (Boophilus) microplus (2008), Comp. Biochem. Physiol. B, 151, 311-316.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.11 additional information nuclear polyphosphates decrease and activity of exopolyphosphatase increases after embryo cellularization until the end of embryogenesis Rhipicephalus microplus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 heparin heparin (0.02 mg/ml) inhibits nuclear and mitochondrial PPX activity in about 90 and 95% Rhipicephalus microplus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 0.0002
-
 (phosphate)3 mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus
3.6.1.11 0.0007
-
 (phosphate)3 nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus
3.6.1.11 0.0009
-
 (phosphate)65 nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus
3.6.1.11 0.0011
-
 (phosphate)15 nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus
3.6.1.11 0.0028
-
 (phosphate)15 mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus
3.6.1.11 0.0036
-
 (phosphate)65 mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 Rhipicephalus microplus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.11 mitochondrion
-
 Rhipicephalus microplus 5739
-
3.6.1.11 additional information existence of 2 different PPX isoforms operating in the nuclei and mitochondria with distinct metal dependence, inhibitor and activator sensitivities Rhipicephalus microplus
-
-
3.6.1.11 nucleus
-
 Rhipicephalus microplus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Co2+ 0.1 mM Co2+, PPX activity is stimulated by a factor of two in the nuclear fraction, but not in the mitochondrial fraction Rhipicephalus microplus
3.6.1.11 Mg2+ 2.5 mM Mg2+, PPX activity is stimulated by a factor of two in the nuclear fraction and in the mitochondrial fraction Rhipicephalus microplus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.11 (phosphate)n + H2O Rhipicephalus microplus regulatory enzyme in polyphosphate metabolism (phosphate)n-1 + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Rhipicephalus microplus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.11 egg segmentation stage, 9th day after oviposition Rhipicephalus microplus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.11 additional information
-
 increasing amounts of total RNA extracted from eggs progressively enhance nuclear PPX activity, whereas it exerts no effect on mitochondrial PPX activity, nuclear PPX activity increases throughout embryogenesis Rhipicephalus microplus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (phosphate)15 + H2O
-
 Rhipicephalus microplus (phosphate)14 + phosphate
-
 ?
3.6.1.11 (phosphate)65 + H2O
-
 Rhipicephalus microplus (phosphate)64 + phosphate
-
 ?
3.6.1.11 (phosphate)n + H2O regulatory enzyme in polyphosphate metabolism Rhipicephalus microplus (phosphate)n-1 + phosphate
-
 ?
3.6.1.11 triphosphate + H2O
-
 Rhipicephalus microplus diphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.11 exopolyphosphatase
-
 Rhipicephalus microplus
3.6.1.11 polyphosphate-phosphohydrolase
-
 Rhipicephalus microplus
3.6.1.11 PPX
-
 Rhipicephalus microplus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.11 30
-
 assay at Rhipicephalus microplus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 7.5
-
 assay at Rhipicephalus microplus