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Literature summary extracted from

  • Friedlos, F.; Lehouritis, P.; Ogilvie, L.; Hedley, D.; Davies, L.; Bermudes, D.; King, I.; Martin, J.; Marais, R.; Springer, C.J.
    Attenuated Salmonella targets prodrug activating enzyme carboxypeptidase G2 to mouse melanoma and human breast and colon carcinomas for effective suicide gene therapy (2008), Clin. Cancer Res., 14, 4259-4266.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.11 medicine CPG2 possesses several properties that make it suitable for suicide gene therapy Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.11 for expression in Salmonella typhimurium cells using the vector pTrc99A Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.11 Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.11 42000
-
 monomer, determined by immunoblotting Pseudomonas sp.
3.4.17.11 83000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.11 additional information Pseudomonas sp. CPG2 cleaves folic acid to pteroic acid and activates a range of bifunctional alkylating agent and antibiotic prodrugs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.11 Pseudomonas sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.11 additional information
-
 0.5 U/ml Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.11 3,5-difluoro-4-[bis(2-iodoethyl)amino]benzoic acid + H2O cognate drug Pseudomonas sp. ?
-
 ?
3.4.17.11 3,5-difluoro-4-[bis(2-iodoethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
3.4.17.11 3-fluoro-4-[bis(2-chloroethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
3.4.17.11 4-[(2-chloroethyl)(2-methylsulphonyloxyethyl)amino]benzoic acid + H2O cognate drug Pseudomonas sp. ?
-
 ?
3.4.17.11 4-[(2-chloroethyl)-(2-methylsulphonyloxyethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
3.4.17.11 4-[bis(2-iodoethyl)-amino]-phenyloxycarbonyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
3.4.17.11 methotrexate + H2O substrate in activity assay Pseudomonas sp. ?
-
 ?
3.4.17.11 additional information CPG2 cleaves folic acid to pteroic acid and activates a range of bifunctional alkylating agent and antibiotic prodrugs Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.11 homodimer
-
 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.4.17.11 carboxypeptidase G2
-
 Pseudomonas sp.
3.4.17.11 CPG2
-
 Pseudomonas sp.