Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuntz, D.A.; Zhong, W.; Guo, J.; Rose, D.R.; Boons, G.J.
    The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A (2009), ChemBioChem, 10, 268-277.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.114
-
 Drosophila melanogaster
3.2.1.114 in complex with inhibitor mannostatin A and its derivatives. The interaction with the backbone carbonyl of residue R876 is crucial to the high potency of the inhibitor and enhanced by the hydrophobic interaction between the thiomethyl group and an aromatic pocket vivinal to the cleavage site Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 (1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol
-
 Drosophila melanogaster
3.2.1.114 (1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol
-
 Drosophila melanogaster
3.2.1.114 (1R,2S,3R,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol
-
 Drosophila melanogaster
3.2.1.114 1-deoxyaminocyclopentitetrol poor inhibitor Drosophila melanogaster
3.2.1.114 Mannostatin A strong inhibitor, reversible, competitive Drosophila melanogaster
3.2.1.114 mannostatin B strong inhibitor, reversible, competitive Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 Golgi apparatus
-
 Drosophila melanogaster 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.114 4-methylumbelliferyl alpha-D-mannopyranoside + H2O Drosophila melanogaster
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Drosophila melanogaster
-
-
-
3.2.1.114 Drosophila melanogaster Q24451 isoform GMII
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 GmII
-
 Drosophila melanogaster
3.2.1.114 Golgi alpha-mannosidase II
-
 Drosophila melanogaster
3.2.1.114 mannose-trimming enzyme
-
 Drosophila melanogaster
3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase II
-
 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 3.8 9 30 min Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.114 0.000036
-
 Mannostatin A
-
 Drosophila melanogaster
3.2.1.114 0.000076
-
 (1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol
-
 Drosophila melanogaster
3.2.1.114 0.00015
-
 mannostatin B
-
 Drosophila melanogaster
3.2.1.114 0.0003
-
 (1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol
-
 Drosophila melanogaster
3.2.1.114 0.0003
-
 (1R,2S,3R,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol
-
 Drosophila melanogaster
3.2.1.114 0.265
-
 1-deoxyaminocyclopentitetrol
-
 Drosophila melanogaster