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Literature summary extracted from

  • Caglio, R.; Valetti, F.; Caposio, P.; Gribaudo, G.; Pessione, E.; Giunta, C.
    Fine-tuning of catalytic properties of catechol 1,2-dioxygenase by active site tailoring (2009), ChemBioChem, 10, 1015-1024.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expression in Escherichia coli BL21 Acinetobacter radioresistens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.1
-
 Acinetobacter baylyi ADP1

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.1 A72D enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
1.13.11.1 A72G enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
1.13.11.1 A72K completely inactive Acinetobacter radioresistens
1.13.11.1 A72N enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
1.13.11.1 A72S enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
1.13.11.1 L69A specificity preference towards 4-chlorocatechol Acinetobacter radioresistens
1.13.11.1 L69G/A72G heavily destabilised Acinetobacter radioresistens
1.13.11.1 additional information catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template Acinetobacter radioresistens
1.13.11.1 additional information used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13 Acinetobacter baylyi ADP1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.000101
-
 4-Chlorocatechol mutant L69A Acinetobacter radioresistens
1.13.11.1 0.00198
-
 4-Chlorocatechol wild-type enzyme Acinetobacter radioresistens
1.13.11.1 0.00204
-
 catechol wild-type enzyme Acinetobacter radioresistens
1.13.11.1 0.00225
-
 3-methylcatechol wild-type enzyme Acinetobacter radioresistens
1.13.11.1 0.00235
-
 catechol mutant A72N Acinetobacter radioresistens
1.13.11.1 0.00242
-
 4-Chlorocatechol mutant L69G/A72G Acinetobacter radioresistens
1.13.11.1 0.00284
-
 4-methylcatechol mutant L69G/A72G Acinetobacter radioresistens
1.13.11.1 0.00576
-
 catechol mutant L69A Acinetobacter radioresistens
1.13.11.1 0.00595
-
 3-methylcatechol mutant L69A Acinetobacter radioresistens
1.13.11.1 0.00613
-
 4-Chlorocatechol mutant A72G Acinetobacter radioresistens
1.13.11.1 0.00654
-
 4-Chlorocatechol mutant A72S Acinetobacter radioresistens
1.13.11.1 0.0093
-
 catechol mutant A72G Acinetobacter radioresistens
1.13.11.1 0.01
-
 4-methylcatechol mutant A72G Acinetobacter radioresistens
1.13.11.1 0.01
-
 catechol mutant A72S Acinetobacter radioresistens
1.13.11.1 0.0128
-
 4-methylcatechol mutant L69A Acinetobacter radioresistens
1.13.11.1 0.0428
-
 catechol mutant L69G/A72G Acinetobacter radioresistens
1.13.11.1 0.0501
-
 4-Chlorocatechol mutant A72N Acinetobacter radioresistens
1.13.11.1 0.053
-
 4-methylcatechol wild-type enzyme Acinetobacter radioresistens
1.13.11.1 0.0691
-
 catechol mutant A72D Acinetobacter radioresistens
1.13.11.1 0.0936
-
 4-methylcatechol mutant A72D Acinetobacter radioresistens
1.13.11.1 0.2081
-
 4-Chlorocatechol mutant A72D Acinetobacter radioresistens
1.13.11.1 0.213
-
 4-methylcatechol mutant A72S Acinetobacter radioresistens
1.13.11.1 0.435
-
 4-methylcatechol mutant A72N Acinetobacter radioresistens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Fe3+ Fe(III)-dependent Acinetobacter radioresistens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter baylyi ADP1 P07773 used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13
-
1.13.11.1 Acinetobacter radioresistens Q9F103 catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template
-
1.13.11.1 Acinetobacter radioresistens S13 Q9F103 catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Acinetobacter radioresistens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
1.13.11.1 3-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4,5-dichlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4,5-dichlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4-chlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4-chlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 4-tert-butylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
1.13.11.1 4-tert-butylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
1.13.11.1 catechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens cis-cis muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 catechol 1,2-dioxygenase
-
 Acinetobacter radioresistens
1.13.11.1 catechol 1,2-dioxygenase
-
 Acinetobacter baylyi ADP1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.1 32.7 51.8 differences between wild-type and mutant enzymes Acinetobacter radioresistens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 6.89 7.49 differs between wild-type and mutant enzymes Acinetobacter radioresistens